A seven-gene-deleted African swine fever virus is safe and effective as a live attenuated vaccine in pigs
A seven-gene-deleted African swine fever virus is safe and effective as a live attenuated vaccine in pigs
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DOI：
10.1007/s11427-020-1657-9
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发表时间：
2020-03-01
影响因子：
9.1
通讯作者：
Bu, Zhigao
中科院分区：
文献类型：
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作者：
Chen, Weiye;Zhao, Dongming;Bu, Zhigao
非洲猪瘟（African Swine Fever，ASF）是一种严重威胁全球养猪业的毁灭性猪传染病。迫切需要一种有效的疫苗。本研究以中国产ASFV HLJ/18为骨架，构建了一系列基因缺失病毒。在无特异性病原猪、商品猪和妊娠母猪中的毒力、免疫原性、安全性和保护效力评价表明，一种名为HLJ/18- 7 GD的病毒，其具有7个基因缺失，在猪中完全减毒，不能转化为强毒株，并且提供对致死ASFV攻击的猪的完全保护。本研究表明HLJ/-18-7GD是一种安全有效的ASFV疫苗，有望在控制ASFV传播中发挥重要作用。
African swine fever (ASF) is a devastating infectious disease in swine that is severely threatening the global pig industry. An efficacious vaccine is urgently required. Here, we used the Chinese ASFV HLJ/18 as a backbone and generated a series of gene-deleted viruses. The virulence, immunogenicity, safety, and protective efficacy evaluation in specific-pathogen-free pigs, commercial pigs, and pregnant sows indicated that one virus, namely HLJ/18-7GD, which has seven genes deleted, is fully attenuated in pigs, cannot convert to the virulent strain, and provides complete protection of pigs against lethal ASFV challenge. Our study shows that HLJ/-18-7GD is a safe and effective vaccine against ASFV, and as such is expected to play an important role in controlling the spread of ASFV.