CONSTRUCTION OF LAC FUSIONS TO THE INDUCIBLE ARGININE AND LYSINE DECARBOXYLASE GENES OF ESCHERICHIA-COLI-K12
CONSTRUCTION OF LAC FUSIONS TO THE INDUCIBLE ARGININE AND LYSINE DECARBOXYLASE GENES OF ESCHERICHIA-COLI-K12
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DOI：
10.1111/j.1365-2958.1989.tb00208.x
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发表时间：
1989-05-01
影响因子：
3.6
通讯作者：
BENNETT, GN
中科院分区：
文献类型：
--
作者：
AUGER, EA;REDDING, KE;BENNETT, GN
多年来，人们已经知道在低 pH 值的厌氧条件下诱导几种氨基酸脱羧酶，但与此类调节相关的机制尚未阐明。为了研究大肠杆菌 K12 的生物降解精氨酸和赖氨酸脱羧酶的调节，分离了这些基因的 Mudlac 融合体。使用脱羧酶指示剂培养基鉴定缺乏赖氨酸脱羧酶或精氨酸脱羧酶的Mudlac融合菌株，并分析它们对β-半乳糖苷酶表达的调节。赖氨酸脱羧酶缺陷菌株中 Mud-lac 融合体的位置已被定位到 CadA 基因的 93.7 分钟，而表现出诱导型精氨酸脱羧酶缺陷的 Mudlac 融合体已被定位到 93.4 分钟。
The induction of several amino acid decarboxylases under anerobic conditions at low pH has been known for many years, but the mechanism associates with this type of regulation has not been elucidated. To study the regulation of the biodegradative arginine and lysine decarboxylases of Escherichia coli K12, Mudlac fusions to these genes were isolated. Mudlac fusion strains deficient for lysine decarboxylase or arginine decarboxylase were identified using decarboxylase indicator media and analysed for their regulation of .beta.-galactosidase expression. The position of the Mud-lac fusion in lysine decarboxylase-deficient strains has been mapped to the CadA gene at 93.7 minutes, while the Mudlac fusions exhibiting a deficiency in the inducible arginine decarboxylase have been mapped to 93.4 minutes.