HIGH-SPEED SEPARATIONS OF DNA SEQUENCING REACTIONS BY CAPILLARY ELECTROPHORESIS

HIGH-SPEED SEPARATIONS OF DNA SEQUENCING REACTIONS BY CAPILLARY ELECTROPHORESIS
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DOI:
10.1021/ac00208a003
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发表时间:
1990-05-01
影响因子:
7.4
通讯作者:
SMITH, LM
SMITH, LM
中科院分区:
化学1区
文献类型:
--
作者:
DROSSMAN, H;LUCKEY, JA;SMITH, LM

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Fluorescently labeled DNA fragments generated in enzymatic sequencing reactions are rapidly separated by capillary gel electrophoresis and detected at attomole levels within the gel-filled capillary. The application of this technology to automated DNA sequence analysis may permit the development of a second generation automated sequencer capable of efficient and cost-effective sequence analysis on the genomic scale.