Atrazine Degradation Using Immobilized Triazine Hydrolase from Arthrobacter aurescens TC1 in Mesoporous Silica Nanomaterials.
Atrazine Degradation Using Immobilized Triazine Hydrolase from Arthrobacter aurescens TC1 in Mesoporous Silica Nanomaterials.
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DOI：
10.1021/acsenvironau.3c00036
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发表时间：
2023-11-15
期刊：
影响因子：
--
通讯作者：
Holz, Richard C
中科院分区：
文献类型：
--
作者：
Diviesti, Karla;Russell-Parks, Glory A;Trewyn, Brian G;Holz, Richard C
首次在介孔二氧化硅纳米材料(MSN)上成功地固定化了来自金色节杆菌TC1(TrzN)的三嗪水解酶。对于未功能化的MSN和用锌(II)功能化的MSN，评价了三种孔径对野生型TrzN的固定化能力：可移动的41号物质(小孔，3 nm孔)、中孔直径为10 nm的介孔二氧化硅纳米材料(MSN-10)(中孔，6-12 nm孔)和扩孔MSN-10(大孔，15-30 nm孔)。在这六种TrZN：MSN生物材料中，TrZN：MSN-10对25℃下50μ的M-阿特拉津的水解液的催化活性最高(3.8±0.4×10-5U/mg)。然后将TrZN：MSN-10生物材料包覆在壳聚糖(TrZN：MSN-10：CHIT)中，因为壳聚糖已被证明在低/高pH、热冲击和有机溶剂存在的极端条件下具有更好的稳定性。TrzN：MSN-10：CHIT是一种优于TrzN：MSN-10的生物材料，因为它在所有储存条件下都表现出更高的活性，在20%的甲醇存在下，在低和高pH值下，以及在高达80°C的高温下。最后，TrzN：MSN-10：CHIT生物材料在河水中表现出完全的活性，这使得它在实际的野外条件下成为一种功能生物材料。这些数据的综合表明，TrzN：MSN-10：CHIT生物材料表现出最好的整体催化性能，使其成为生物修复阿特拉津的一种有前景的生物催化剂。
Triazine hydrolase fromArthrobacter aurescens TC1 (TrzN) was successfully immobilized on mesoporous silica nanomaterials (MSNs) for the first time. For both nonfunctionalized MSNs and MSNs functionalized with Zn(II), three pore sizes were evaluated for their ability to immobilize wild-type TrzN: Mobile composition of matter no. 41 (small, 3 nm pores), mesoporous silica nanoparticle material with 10 nm pore diameter (MSN-10) (medium, 6–12 nm pores), and pore-expanded MSN-10 (large, 15–30 nm pores). Of these six TrzN:MSN biomaterials, it was shown that TrzN:MSN-10 was the most active (3.8 ± 0.4 × 10–5 U/mg) toward the hydrolysis of a 50 μM atrazine solution at 25 °C. The TrzN:MSN-10 biomaterial was then coated in chitosan (TrzN:MSN-10:Chit) as chitosan has been shown to increase stability in extreme conditions such as low/high pH, heat shock, and the presence of organic solvents. TrzN:MSN-10:Chit was shown to be a superior TrzN biomaterial to TrzN:MSN-10 as it exhibited higher activity under all storage conditions, in the presence of 20% MeOH, at low and high pH values, and at elevated temperatures up to 80 °C. Finally, the TrzN:MSN-10:Chit biomaterial was shown to be fully active in river water, which establishes it as a functional biomaterial under actual field conditions. A combination of these data indicate that the TrzN:MSN-10:Chit biomaterial exhibited the best overall catalytic profile making it a promising biocatalyst for the bioremediation of atrazine.
影响因子：
8.4
作者：
Darby JF;Atobe M;Firth JD;Bond P;Davies GJ;O'Brien P;Hubbard RE
通讯作者：
Hubbard RE