Role of the Nanos Homolog During Sea Urchin Development
Role of the Nanos Homolog During Sea Urchin Development
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DOI：
10.1002/dvdy.22074
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发表时间：
2009-10-01
影响因子：
2.5
通讯作者：
Yamamoto, Takashi
中科院分区：
文献类型：
--
作者：
Fujii, Takayoshi;Sakamoto, Naoaki;Yamamoto, Takashi
nanos基因在动物原始生殖细胞的发育过程中起着重要作用。在海胆，Hemicentrotus pulcherrimus，小micromere后代特异性表达HpNanos mRNA，这种表达继续在左体腔袋，产生的主要组成部分的成人雏形。在这项研究中，我们表明，吗啉敲低的HpNanos导致延迟的初级间充质细胞的侵入和减少的细胞数量，包括左体腔袋。嵌合体和整个胚胎的敲除分析显示，从原肠尖的小micromere后代的消失。此外，在HpNanos敲除和微粒体缺失的胚胎中，HpNanos mRNA的表达在其他细胞系中被诱导。两者合计，我们的研究结果表明，HpNanos是参与小micromere后代与其他细胞谱系的诱导相互作用，并且HpNanos是小micromere后代的生存所必需的。发展动力学238：2511-2521，2009年。(c)2009威利-利斯公司
The nanos genes play important roles in the development of primordial germ cells in animal species. In the sea urchin, Hemicentrotus pulcherrimus, small micromere descendants specifically express HpNanos mRNA and this expression continues in the left coelomic pouch, which produces the major component of the adult rudiment. In this study, we showed that morpholino knockdown of HpNanos resulted in a delay of primary mesenchyme cell ingression and a decrease in the number of cells comprising the left coelomic pouch. Knockdown analysis in chimeras and whole embryos revealed the disappearance of small micromere descendants from the archenteron tip. Furthermore, the expression of HpNanos mRNA was induced in other cell lineages in the HpNanos-knockdown and micromere-deleted embryos. Taken together, our results suggest that HpNanos is involved in the inductive interaction of small micromere descendants with other cell lineages, and that HpNanos is required for the survival of small micromere descendants. Developmental Dynamics 238:2511-2521, 2009. (c) 2009 Wiley-Liss, Inc.