High level expression of human epithelial beta-defensins (hBD-1, 2 and 3) in papillomavirus induced lesions.
High level expression of human epithelial beta-defensins (hBD-1, 2 and 3) in papillomavirus induced lesions.
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DOI：
10.1186/1743-422x-3-75
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发表时间：
2006-09-08
期刊：
影响因子：
4.8
通讯作者：
Schweinfurth, John M.
中科院分区：
文献类型：
--
作者：
Chong, Kong T.;Xiang, Liangbin;Wang, Xiaohong;Jun, Eunjoo L.;Xi, Long-fu;Schweinfurth, John M.
上皮防御素（Epithelial defensins）包括人β防御素（human β-defensins，hBD）和α防御素（human α-defensins，HDs），是一类在粘膜防御系统中发挥重要作用的抗菌肽。然而，防御素在乳头瘤病毒诱导的上皮病变中的作用尚不清楚。从15例复发性呼吸道乳头状瘤病（RRP）患者中前瞻性收集乳头状瘤组织，并通过定量逆转录聚合酶链反应（RT-PCR）分析防御素和趋化因子IL-8的表达。所有RRP患者的乳头状瘤样本中均可检测到HBD-1、-2和-3 mRNA，且水平高于健康个体的正常口腔粘膜组织。免疫组化显示hBD-1和2均定位于乳头状瘤组织的上上皮层。散点图分析显示hBD-2和hBD-3的表达似乎相关（r = 0.837，p < 0.01），表明它们在乳头瘤病毒诱导的病变中是共诱导的。与hBD不同，在乳头状瘤和口腔粘膜中仅检测到低水平的HD 5和HD 6。人β-防御素在呼吸道乳头状瘤中上调。这一新的发现表明，hBD可能有助于针对乳头状瘤病毒诱导的上皮病变的先天性和适应性免疫反应。
Epithelial defensins including human β-defensins (hBDs) and α-defensins (HDs) are antimicrobial peptides that play important roles in the mucosal defense system. However, the role of defensins in papillomavirus induced epithelial lesions is unknown. Papilloma tissues were prospectively collected from 15 patients with recurrent respiratory papillomatosis (RRP) and analyzed for defensins and chemokine IL-8 expression by quantitative, reverse-transcriptase polymerase chain reaction (RT-PCR) assays. HBD-1, -2 and -3 mRNAs were detectable in papilloma samples from all RRP patients and the levels were higher than in normal oral mucosal tissues from healthy individuals. Immunohistochemical analysis showed that both hBD-1 and 2 were localized in the upper epithelial layers of papilloma tissues. Expression of hBD-2 and hBD-3 appeared to be correlated as indicated by scatter plot analysis (r = 0.837, p < 0.01) suggesting that they were co-inducible in papillomavirus induced lesions. Unlike hBDs, only low levels of HD5 and HD6 were detectable in papillomas and in oral mucosa. Human β-defensins are upregulated in respiratory papillomas. This novel finding suggests that hBDs might contribute to innate and adaptive immune responses targeted against papillomavirus-induced epithelial lesions.