Neuropeptide S increases motor activity and thermogenesis in the rat through sympathetic activation
Neuropeptide S increases motor activity and thermogenesis in the rat through sympathetic activation
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DOI：
10.1016/j.npep.2017.04.005
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发表时间：
2017-10-01
期刊：
影响因子：
2.9
通讯作者：
Murakami, Noboru
中科院分区：
文献类型：
--
作者：
Ensho, Takuya;Nakahara, Keiko;Murakami, Noboru
神经肽S被认为是GPR154的内源性配体，现被命名为神经肽S受体，其中枢作用尚未完全阐明。我们研究了NPS对大鼠体温、能量消耗、运动活动和肾上腺激素分泌的中枢作用。脑室注射NPS使体温升高，呈剂量依赖关系。冰冻注射NPS也显著增加了能量消耗和运动活动。此外，冰冻注射NPS可增加外周血中肾上腺素和皮质酮的浓度。预先给予β1和β2肾上腺素能受体阻断剂噻吗洛尔可抑制NPS引起的体温升高。脑干NPS和下丘脑NPSR的表达均呈昼夜节律，峰值出现在暗期的上半段。为了研究内源性NPS是否参与体温调节，在黑暗后1小时给予NPSR拮抗剂SHA68。SHA68可减弱夜间体温升高。这些结果表明，NPS参与了交感神经系统的调节。
The central role of neuropeptide S (NPS), identified as the endogenous ligand for GPR154, now named neuropeptide S receptor (NPSR), has not yet been fully clarified. We examined the central role of NPS for body temperature, energy expenditure, locomotor activity and adrenal hormone secretion in rats. Intracerebroventricular (icv) injection of NPS increased body temperature in a dose-dependent manner. Energy consumption and locomotor activity were also significantly increased by icy injection of NPS. In addition, icy injection of NPS increased the peripheral blood concentration of adrenalin and corticosterone. Pretreatment with the beta 1- and beta 2-adrenergic receptor blocker timolol inhibited the NPS-induced increase of body temperature. The expression of both NPS mRNA in the brainstem and NPSR mRNA in the hypothalamus showed a nocturnal rhythm with a peak occurring during the first half of the dark period. To examine whether the endogenous NPS is involved in regulation of body temperature, NPSR antagonist SHA68 was administered one hour after darkness. SHA68 attenuated the nocturnal rise of body temperature. These results suggest that NPS contributes to the regulation of the sympathetic nervous system.