Measurement of serum Anti-Mullerian hormone by Beckman Coulter ELISA and DSL ELISA:: Comparison and relevance in Assisted Reproduction Technology (ART)
Measurement of serum Anti-Mullerian hormone by Beckman Coulter ELISA and DSL ELISA:: Comparison and relevance in Assisted Reproduction Technology (ART)
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DOI：
10.1016/j.cca.2006.06.013
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发表时间：
2007-01-01
影响因子：
5
通讯作者：
Masson, Damien
中科院分区：
文献类型：
--
作者：
Freour, Thomas;Mirallie, Sophie;Masson, Damien
背景：大量研究表明，在辅助生殖技术周期中，血清AMH水平可作为预测卵巢反应和妊娠的临床指标。方法：我们比较了两种商业免疫分析方法(AMH Beckman Coulter EL ISA和AMH DSL EL ISA)，并评估了血清AMH水平作为辅助生殖技术周期中卵巢反应和妊娠的预测价值。结果：我们发现两种方法之间存在密切的线性关系，但DSL试剂盒的AMH水平几乎比Beckman Coulter试剂盒低4.6倍。我们发现两种方法获得的成熟卵母细胞数与AMH水平呈显著正相关。无论采用何种方法，妊娠组与非妊娠组的AMH水平均无显著差异。事实上，使用Beckman Coulter方法，所有孕妇的血清AMH水平都超过1.4mU g/L。相反，DSL试剂盒没有找到临界值。结论：我们的结果首次清楚地表明，即使两种方法之间的相关性仍然很好，AMH结果仍然与方法有关。Beckman Coulter AMH酶联免疫吸附试验在用于辅助生殖患者的预后时应产生临床一致性。(C)2006爱思唯尔B.V.保留所有权利。
Background: Numerous studies have evocated the clinical usefulness of serum AMH levels as a predictor of ovarian response and pregnancy in assisted reproductive technology cycles. Nevertheless, the analysis of the literature shows a great dispersion in serum AMH concentrations obtained with different methods from almost comparable populations.Methods: We compared two commercial immunoassays (AMH Beckman Coulter ELISA and AMH DSL ELISA) and we evaluated the AMH levels in serum as a prognosis value for ovarian response and pregnancy in assisted reproductive technology cycles.Results: We found a close linear relationship between the two methods but AMH levels were almost 4.6-fold lower with the DSL kit than with the Beckman Coulter kit. We found a significant and positive correlation between the number of mature ovocytes inseminated and AMH levels obtained with the two methods. Whatever the ELISA used, we found no significant difference between AMH level of pregnancy and non pregnancy groups. Indeed, using the Beckman Coulter method, all pregnant patients had serum AMH levels over 1.4 mu g/L. Conversely, no cut-off value can be found for the DSL kit.Conclusion: Our results show clearly for the first time that AMH results are method dependent even if the correlation obtained between the two methods remained excellent. The Beckman Coulter AMH ELISA should produce clinical agreement when used for prognosis purposes on patients undergoing assisted reproduction. (c) 2006 Elsevier B.V. All rights reserved.