Heavy-chain variable regions in carcharhine sharks: development of a comprehensive model for the evolution of VH domains among the gnathanstomes.
Heavy-chain variable regions in carcharhine sharks: development of a comprehensive model for the evolution of VH domains among the gnathanstomes.
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真鲨重链可变区：颌口动物 VH 结构域进化综合模型的开发。
DOI：
10.1038/icb.1996.63
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发表时间：
1996
影响因子：
4
通讯作者：
Marchalonis,JJ
中科院分区：
文献类型：
--
作者：
Shen,SX;Bernstein,RM;Schluter,SF;Marchalonis,JJ
我们确定了编码沙洲鲨和牛鲨 VH 区的 18 个 DNA 克隆的序列。所有这些序列均表现出高等脊椎动物已知 VH 基因的关键结构编码特征。这些VH序列表现出相当大的多样性，按照80％DNA序列同一性的标准可分为6个家族。一些 VH 基因克隆与两个或多个家族的重叠是真鲨中发现的一个特殊特征，这表明 VH 多样化是一个持续的过程。在所有代表性颌口动物和鲨鱼重免疫球蛋白 igW 的 VH 中发现的重链 V 区的基本序列模式为选择所有 VH 结构中的规范残基提供了证据，软骨鱼类 VH 序列可分为两类或族，一类包含“经典”VH 集，另一类包含与 IgW 相关的 VH (Vω)。系统发育分析将 VH 簇作为所有经典 VH 的根，并表明 Vω 集很可能是原始重链的集。
We determined the sequence of 18 DNA clones encoding VHregions of sandbar shark and bull shark. All of these sequences exhibit key structural coding features characteristic of known VHgenes of higher vertebrates. These VHsequences disclosed considerable diversity, and can be divided into six families according to the criterion of 80% DNA sequence identity. The overlapping of some VHgene clones to two or more families is a particular feature found in carcharhine sharks, which suggests that VHdiversification is a continuing process. The basic sequence patterns of heavy‐chain V regions found in all representative gnathanstomes and in VHof the shark heavy immunoglobulin igW provides evidence for selection of canonical residues in all VHstructures, Elasmobranch VHsequences can be divided into two classes or clans, one comprising the ‘classical’ VHset and the other comprising VHs related to those of IgW (Vω). Phylogenetic analyses place the VHcluster as the root of all the classic VHs and indicates that the Vω set is most probably that of the primordial heavy chain.