Delayed animal aging through the recovery of stem cell senescence by platelet rich plasma
Delayed animal aging through the recovery of stem cell senescence by platelet rich plasma
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DOI：
10.1016/j.biomaterials.2014.08.034
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发表时间：
2014-12-01
期刊：
影响因子：
14
通讯作者：
Deng, Win-Ping
中科院分区：
文献类型：
--
作者：
Liu, Hen-Yu;Huang, Chiung-Fang;Deng, Win-Ping
衰老与功能性干细胞的丧失以及组织和器官再生潜力的丧失有关。此前，我们证明，富含血小板血浆（PRP）/胚胎成纤维细胞移植后，卵巢切除加速衰老小鼠（OVX-SAMP8）的寿命显着延长，并且与同源抗衰老品系小鼠的寿命相似。本研究的目的是探讨 PRP 恢复衰老细胞潜力并延缓动物衰老的潜力。我们首先检查了与年轻小鼠相比，老年小鼠的干细胞是否会衰老。从年轻和老年小鼠身上采集原代脂肪干细胞（ADSC）和骨髓干细胞（BMSC），发现细胞衰老与动物衰老密切相关。随后，我们证明PRP可以从衰老中恢复细胞潜力，例如促进细胞生长（细胞增殖和集落形成）、增加成骨、减少脂肪生成、恢复细胞衰老相关标志物以及抵抗衰老小鼠干细胞的氧化应激。结果还表明，与老年对照小鼠的结果相比，PRP 治疗老年小鼠可以延缓小鼠的衰老，如存活率、体重和衰老表型（行为和总体形态），恢复其干细胞的细胞潜力。总之，这些发现表明，PRP 有潜力通过恢复干细胞衰老来延缓衰老，并可用作组织再生和未来复兴的替代药物。 (C) 2014 Elsevier Ltd. 保留所有权利。
Aging is related to loss of functional stem cell accompanying loss of tissue and organ regeneration potentials. Previously, we demonstrated that the life span of ovariectomy-senescence accelerated mice (OVX-SAMP8) was significantly prolonged and similar to that of the congenic senescence-resistant strain of mice after platelet rich plasma (PRP)/embryonic fibroblast transplantation. The aim of this study is to investigate the potential of PRP for recovering cellular potential from senescence and then delaying animal aging. We first examined whether stem cells would be senescent in aged mice compared to young mice. Primary adipose derived stem cells (ADSCs) and bone marrow derived stem cells (BMSCs) were harvested from young and aged mice, and found that cell senescence was strongly correlated to animal aging. Subsequently, we demonstrated that PRP could recover cell potential from senescence, such as promote cell growth (cell proliferation and colony formation), increase osteogenesis, decrease adipogenesis, restore cell senescence related markers and resist the oxidative stress in stem cells from aged mice. The results also showed that PRP treatment in aged mice could delay mice aging as indicated by survival, body weight and aging phenotypes (behavior and gross morphology) in term of recovering the cellular potential of their stem cells compared to the results on aged control mice. In conclusion these findings showed that PRP has potential to delay aging through the recovery of stem cell senescence and could be used as an alternative medicine for tissue regeneration and future rejuvenation. (C) 2014 Elsevier Ltd. All rights reserved.