A synthetic intron in a naturally intronless yeast pre-tRNA is spliced efficiently in vivo.
A synthetic intron in a naturally intronless yeast pre-tRNA is spliced efficiently in vivo.
复制标题
天然无内含子酵母前 tRNA 中的合成内含子在体内有效剪接。
DOI：
10.1128/mcb.9.1.329-331.1989
复制
发表时间：
1989
影响因子：
5.3
通讯作者：
Culbertson,MR
中科院分区：
文献类型：
--
作者：
Winey,M;Edelman,I;Culbertson,MR
酿酒酵母谷氨酰胺tRNACAG由一个无内含子的单拷贝基因SUP60编码。我们通过在SUP60基因中插入一个合成内含子，对这种tRNA的生物合成提出了剪接要求。遗传分析表明，中断的基因在体内产生功能成熟的tRNA产物。
Saccharomyces cerevisiaeglutamine tRNACAGis encoded by an intronless, single-copy gene,SUP60. We have imposed a requirement for splicing in the biosynthesis of this tRNA by inserting a synthetic intron in theSUP60gene. Genetic analysis demonstrated that the interrupted gene produces a functional, mature tRNA product in vivo.