Simultaneous submicrometric 3D imaging of the micro-vascular network and the neuronal system in a mouse spinal cord.
Simultaneous submicrometric 3D imaging of the micro-vascular network and the neuronal system in a mouse spinal cord.
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DOI：
10.1038/srep08514
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发表时间：
2015-02-17
影响因子：
4.6
通讯作者：
Cedola A
中科院分区：
文献类型：
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作者：
Fratini M;Bukreeva I;Campi G;Brun F;Tromba G;Modregger P;Bucci D;Battaglia G;Spanò R;Mastrogiacomo M;Requardt H;Giove F;Bravin A;Cedola A
脊髓血管(VN)和神经元网络的缺陷是导致严重的神经退行性病变的原因。由于研究工具的不足，缺乏对VN和神经系统的完整精细结构的了解是一个关键问题。传统的2D成像产生不完整的空间覆盖，导致可能的数据误解，而标准的3D计算机断层成像获得的分辨率和对比度不足。我们发现，X射线高分辨率相衬断层成像可以同时显示体外小鼠脊髓的三维VN和神经元系统，范围从毫米到数百纳米，既不使用造影剂，也不进行切片，也不需要破坏性的样品制备。我们对微血管网络和微细神经纤维、轴突束和神经元胞体的三维分布进行了成像。我们的方法非常适合于神经退行性病变和脊髓损伤的临床前研究，特别是解决VN和神经系统之间的纠缠关系。
Faults in vascular (VN) and neuronal networks of spinal cord are responsible for serious neurodegenerative pathologies. Because of inadequate investigation tools, the lacking knowledge of the complete fine structure of VN and neuronal system represents a crucial problem. Conventional 2D imaging yields incomplete spatial coverage leading to possible data misinterpretation, whereas standard 3D computed tomography imaging achieves insufficient resolution and contrast. We show that X-ray high-resolution phase-contrast tomography allows the simultaneous visualization of three-dimensional VN and neuronal systems of ex-vivo mouse spinal cord at scales spanning from millimeters to hundreds of nanometers, with nor contrast agent nor sectioning and neither destructive sample-preparation. We image both the 3D distribution of micro-capillary network and the micrometric nerve fibers, axon-bundles and neuron soma. Our approach is very suitable for pre-clinical investigation of neurodegenerative pathologies and spinal-cord-injuries, in particular to resolve the entangled relationship between VN and neuronal system.