Genetic diversity analysis and conservation of the endangered Chinese endemic herb Dendrobium officinale Kimura et Migo (Orchidaceae) based on AFLP
Genetic diversity analysis and conservation of the endangered Chinese endemic herb Dendrobium officinale Kimura et Migo (Orchidaceae) based on AFLP
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DOI：
10.1007/s10709-007-9196-8
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发表时间：
2008-06-01
期刊：
影响因子：
1.5
通讯作者：
Gu, Sun
中科院分区：
文献类型：
--
作者：
Li, Xuexia;Ding, Xiaoyu;Gu, Sun
铁皮石斛是中国特有的一种濒危多年生草本植物。确定该物种的遗传多样性水平和种群遗传结构格局将有助于该物种的保护和管理。采用扩增片段长度多态(AFLP)方法，对12个群体的遗传多样性和群体结构进行了分析。POPGENE检测到较高水平的遗传多样性(H-E=0.269)。方差分析表明，群体间存在中等变异，群体间的PHi(ST)值在0.047~0.578之间，平均有26.97%的遗传变异发生在群体间。用热重分析法(TFPGA)在UPGMA树状图上显示了三个主要簇团，这与NTsys的主坐标分析(PCO)结果一致。维持稳定的环境对珍稀濒危植物的就地保护和管理至关重要，而对于迁地保护来说，设计一个完整的种质资源库是非常重要的。
Dendrobium officinale is a critically endangered perennial herb endemic to China. Determining the levels of genetic diversity and patterns of population genetic structure of this species would assist in its conservation and management. Data of 12 populations were used to assess its genetic diversity and population structure, employing the method of amplified fragment length polymorphism (AFLP). A high level of genetic diversity was detected (H-E = 0.269) with POPGENE. As revealed by AMOVA analysis, there was moderate variation between pairs of populations with Phi(ST) values ranging from 0.047 to 0.578 and on average 26.97% of the genetic variation occurred among populations. Three main clusters were shown in UPGMA dendrogram using TFPGA, which is consistent with the result of principal coordinate ananlysis (PCO) using NTSYS. Keeping a stable environment is critical for the in situ conservation and management of this rare and endangered plant, and for ex situ conservation it is important to design an integrated germplasm bank.