Down-regulation of hypothalamic 5-HT1A receptors in morphine-abstinent rats.
Down-regulation of hypothalamic 5-HT1A receptors in morphine-abstinent rats.
复制标题
吗啡戒断大鼠下丘脑 5-HT1A 受体的下调。
DOI：
10.1016/0014-2999(90)90284-d
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发表时间：
1990
影响因子：
5
通讯作者：
Bhargava,HN
中科院分区：
文献类型：
--
作者：
Gulati,A;Bhargava,HN
本实验观察了吗啡耐受-依赖和戒断对大鼠脑区和脊髓5-HT_（1A）受体的影响。在雄性Sprague-Dawley大鼠中通过在7天期间植入6个吗啡丸（每个丸含有75 mg吗啡游离碱）来诱导对吗啡的耐受性和身体依赖性。使用两组大鼠进行结合研究。在一组中，颗粒保持完整（耐受依赖性），而在另一组中，它们被移除（禁欲）。处死大鼠，切除脊髓和大脑。将脑解剖成七个区域（杏仁核、海马、下丘脑、纹状体、中脑、脑桥+髓质和皮质）。以[~ 3 H]8-羟基-二-正丙基氨基四氢萘（[~ 3 H]DPAT）为配体，未标记的5-HT为非特异性结合，对5-HT_（1A）受体进行了表征。在吗啡和安慰剂耐受依赖大鼠中，[3 H]DPAT与脑区和脊髓中5-HT 1A受体的结合没有差异。吗啡戒断大鼠下丘脑[~ 3 H]DPAT的Bmax值下降61.9%。在其他脑区和脊髓中未观察到Bmax值变化。Kd值未受影响。DPAT皮下给药产生低体温的大鼠从丸已被删除。DPAT诱导的低温反应的强度更大的吗啡戒断大鼠相比，安慰剂戒断大鼠。由于DPAT主要作用于突触前5-HT神经元，因此，吗啡戒断大鼠下丘脑5-HT_（1A）受体表达下调，而吗啡耐受大鼠下丘脑5-HT_（1A）受体表达不受影响。
The effects of morphine tolerance-dependence and abstinence on 5-HT1Areceptors in brain regions and spinal cord of the rat were determined. Tolerance to and physical dependence on morphine was induced in male Sprague-Dawley rats by implanting six morphine pellets (each containing 75 mg of morphine free base) during a seven day period. Two groups of rats were used for binding studies. In one group the pellets were left intact (tolerant-dependent) and in the other they were removed (abstinent). Rats were killed, and spinal cords and brains were excised. Brain was dissected into seven regions (amygdala, hippocampus, hypothalamus, striatum, midbrain, pons + medulla and cortex). 5-HT1Areceptors were characterized by using [3H]8-hydroxy-di-n-propylaminotetralin ([3H]DPAT) as the ligand and unlabelled 5-HT to determine the non-specific binding. In morphine and placebo tolerant-dependent rats the binding of [3H]DPAT to 5-HT1Areceptors in brain regions and spinal cord did not differ. The Bmaxvalue of [3H]DPAT in the hypothalamus of morphine abstinent rats was decreased by 61.9%. No change in Bmaxvalue was observed in other brain regions and spinal cord. The Kdvalues were unaffected. Subcutaneous administration of DPAT produced hypothermia in rats from which pellets had been removed. The intensity of DPAT-induced hypothermic response was greater in morphine abstinent rats as compared to placebo abstinent rats. Since DPAT is believed to have a major action on the presynaptic 5-HT neurons, it is concluded that in morphine abstinent rats 5-HT1Areceptors are down-regulated in hypothalamus, but in morphine tolerant-dependent rats they are unaffected.
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耐受和身体依赖性大鼠吗啡戒断后，用 [3H]SCH 23390 标记的脑和脊髓多巴胺 D1 受体的修饰。
DOI：
--
发表时间：
1990
期刊：
The Journal of pharmacology and experimental therapeutics
影响因子：
--
作者：
Bhargava,HN;Gulati,A
通讯作者：
Gulati,A
DOI：
--
发表时间：
1981-08
期刊：
The Journal of pharmacology and experimental therapeutics
影响因子：
--
作者：
H. Bhargava
通讯作者：
H. Bhargava
大鼠体内颗粒植入诱导的吗啡耐受性的定量
影响因子：
3.3
作者：
H. Bhargava
通讯作者：
H. Bhargava
大鼠中枢神经系统中血清素敏感的腺苷酸环化酶和[3]血清素结合位点—I：动力学参数和药理学特性
影响因子：
5.8
作者：
D. Nelson;A. Herbet;A. Enjalbert;J. Bockaert;M. Hamon
通讯作者：
M. Hamon
对吗啡的身体依赖无法增加大鼠大脑中的血清素周转率。
影响因子：
5.8
作者：
S. Algeri;E. Costa
通讯作者：
E. Costa