MEMORY B-CELLS FROM HUMAN TONSILS COLONIZE MUCOSAL EPITHELIUM AND DIRECTLY PRESENT ANTIGEN TO T-CELLS BY RAPID UP-REGULATION OF B7-1 AND B7-2
MEMORY B-CELLS FROM HUMAN TONSILS COLONIZE MUCOSAL EPITHELIUM AND DIRECTLY PRESENT ANTIGEN TO T-CELLS BY RAPID UP-REGULATION OF B7-1 AND B7-2
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DOI：
10.1016/1074-7613(95)90048-9
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发表时间：
1995-03-01
期刊：
影响因子：
32.4
通讯作者：
BANCHEREAU, J
中科院分区：
文献类型：
--
作者：
LIU, YJ;BARTHELEMY, C;BANCHEREAU, J
通过IgD（+）初始α细胞和CD 38（+）生殖中心B细胞和浆细胞的阴性选择，从扁桃体中分离携带突变IgV区基因的人记忆B细胞。它们主要存在于人扁桃体的上皮内，而不在B细胞滤泡中。由于辅助分子B7-1/CD 80和B7-2/CD 86的组成型表达，记忆a细胞而不是幼稚B细胞具有直接向T细胞呈递抗原的能力。通过抗原受体和CD 40抗原的信号导致这两种分子在记忆A细胞上比在幼稚B细胞上更迅速和更强烈地进一步上调。粘膜表面下记忆B细胞的独特解剖学定位，连同它们的强APC能力，可以解释众所周知的迅速和稳健的二次抗体应答。
Human memory B cells that carry mutated IgV region genes were isolated from tonsils by negative selection of IgD(+) naive a cells and CD38(+) germinal center B cells and plasma cells. They were mainly found within the intraepithelial areas, but not in the B cell follicles of human tonsils. Memory a cells but not naive B cells have the capacity to present antigen directly to T cells, owing to the constitutive expression of the accessory molecules B7-1/CD80 and B7-2/CD86. Signals through antigen receptors and CD40 antigen result in these two molecules being further up-regulated more rapidly and strongly on memory a cells than on naive B cells. The unique anatomical localization of memory B cells beneath the surface of mucosa, together with their strong APC capacity, may explain the well-known prompt and robust secondary antibody responses.