Taurine protects rat bronchioles from acute ozone-induced lung inflammation and hyperplasia.
Taurine protects rat bronchioles from acute ozone-induced lung inflammation and hyperplasia.
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DOI：
10.3109/01902149509031768
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发表时间：
1995-11
影响因子：
1.7
通讯作者：
G. Schuller-Levis;Ronald E. Gordon;E. Park;K. Pendino;Debra L. Laskin
中科院分区：
文献类型：
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作者：
G. Schuller-Levis;Ronald E. Gordon;E. Park;K. Pendino;Debra L. Laskin
臭氧是一种强烈的呼吸道刺激物，已知会导致人类和实验动物的肺损伤。目前的研究确定，如果臭氧引起的肺部炎症是修改预处理动物牛磺酸，解毒抗氧化剂。在暴露于2ppm臭氧3小时之前，大鼠在其饮用水中用5%牛磺酸预处理10天（对照组仅接受水）。在臭氧暴露后2，6，12，24，48和72小时，麻醉大鼠，肺灌注固定的组织病理学评价。另一组大鼠用于检查支气管肺泡灌洗细胞计数和羟脯氨酸水平。臭氧暴露后48小时的支气管肺泡灌洗液细胞计数显示，总炎症细胞和更少的多形核白细胞伴随着减少羟脯氨酸在灌洗液中的臭氧暴露大鼠与牛磺酸预处理的大鼠相比，没有收到牛磺酸。光学显微镜检查显示，在大鼠暴露于臭氧的肺部炎症细胞浸润。随后在终末和呼吸性细支气管中出现局灶性增生。大鼠预处理牛磺酸，然后暴露于臭氧显示没有这些变化。此外，虽然有一个显着的减少细胞增殖的DNA前体掺入在肺中的大鼠预处理牛磺酸臭氧暴露前相比，未补充大鼠，标记细胞的分布是相同的牛磺酸补充和未补充组。此外，与仅接受水的大鼠相比，在用饮食牛磺酸预处理的大鼠的血浆、全血和灌洗液中发现显著更高水平的牛磺酸。结果表明，补充牛磺酸保护大鼠肺上皮急性臭氧诱导的肺部炎症和增生。
Ozone is a potent respiratory irritant known to induce lung injury in humans and experimental animals. The present studies determined if ozone-induced lung inflammation was modified by pretreatment of animals with taurine, a detoxifying antioxidant. Rats were pretreated for 10 days with 5% taurine in their drinking water (controls received water only) prior to exposure to 2 ppm ozone for 3 h. At 2, 6, 12, 24, 48, and 72 h after ozone exposure, rats were anesthetized and the lungs were perfusion-fixed for histopathologic evaluation. An additional group of rats was used to examine bronchoalveolar lavage cell counts and hydroxyproline levels. A count of bronchoalveolar lavage cells 48 h after ozone exposure showed significantly fewer total inflammatory cells and fewer polymorphonuclear leukocytes accompanied by a reduction in hydroxyproline in the lavage fluid of ozone-exposed rats pretreated with taurine compared to rats that did not receive taurine. Light microscopy revealed an inflammatory cell infiltrate in the lungs of rats exposed to ozone. This was followed by focal hyperplasia in the terminal and respiratory bronchioles. Rats pretreated with taurine and then exposed to ozone showed none of these alterations. In addition, although there was a significant reduction in cell proliferation as measured by DNA precursor incorporation in the lungs of rats pretreated with taurine prior to ozone exposure compared to unsupplemented rats, the distribution of labeled cells was the same in taurine supplemented and unsupplemented groups. Also, significantly higher levels of taurine were found in the plasma, whole blood, and lavage fluid of rats pretreated with dietary taurine compared to rats that received water only. The results suggest that supplemental taurine protects rat lung epithelium from acute ozone-induced lung inflammation and hyperplasia.