COX16 promotes COX2 metallation and assembly during respiratory complex IV biogenesis.
COX16 promotes COX2 metallation and assembly during respiratory complex IV biogenesis.
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DOI：
10.7554/elife.32572
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发表时间：
2018-01-30
期刊：
影响因子：
7.7
通讯作者：
Rehling P
中科院分区：
文献类型：
--
作者：
Aich A;Wang C;Chowdhury A;Ronsör C;Pacheu-Grau D;Richter-Dennerlein R;Dennerlein S;Rehling P
线粒体氧化磷酸化系统的细胞色素c氧化酶通过氧化还原当量衍生电子减少分子氧。保守的线粒体编码的COX1-和cox -亚基是含有血红素和铜中心的核心亚基，它们催化水的形成。COX1和COX2最初遵循独立的生物发生途径，产生具有亚基特异性的、伴侣样的组装因子的组装模块，帮助氧化还原中心的形成。在这里，我们发现细胞色素c氧化酶组装所需的蛋白质COX16与新合成的COX2及其铜中心形成的金属伴侣SCO1， SCO2和COA6特异性相互作用。SCO1向cox - 2模块的募集是依赖于cox - 16的，并且SCO1中的患者模拟突变影响与cox - 16的相互作用。这些发现暗示COX16参与了cua位点的形成。令人惊讶的是，COX16也存在于含有COX1的组装中间体和COX2招募到COX1中。我们得出结论，COX16参与了COX1和COX2装配线的合并。
Cytochrome c oxidase of the mitochondrial oxidative phosphorylation system reduces molecular oxygen with redox equivalent-derived electrons. The conserved mitochondrial-encoded COX1- and COX2-subunits are the heme- and copper-center containing core subunits that catalyze water formation. COX1 and COX2 initially follow independent biogenesis pathways creating assembly modules with subunit-specific, chaperone-like assembly factors that assist in redox centers formation. Here, we find that COX16, a protein required for cytochrome c oxidase assembly, interacts specifically with newly synthesized COX2 and its copper center-forming metallochaperones SCO1, SCO2, and COA6. The recruitment of SCO1 to the COX2-module is COX16- dependent and patient-mimicking mutations in SCO1 affect interaction with COX16. These findings implicate COX16 in CuA-site formation. Surprisingly, COX16 is also found in COX1-containing assembly intermediates and COX2 recruitment to COX1. We conclude that COX16 participates in merging the COX1 and COX2 assembly lines.