Lactic Acid Promotes Cell Survival by Blocking Autophagy of B16F10 Mouse Melanoma Cells under Glucose Deprivation and Hypoxic Conditions
Lactic Acid Promotes Cell Survival by Blocking Autophagy of B16F10 Mouse Melanoma Cells under Glucose Deprivation and Hypoxic Conditions
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DOI：
10.1248/bpb.b18-00919
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发表时间：
2019-05-01
影响因子：
2
通讯作者：
Sadzuka, Yasuyuki
中科院分区：
文献类型：
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作者：
Matsuo, Taisuke;Daishaku, Shun;Sadzuka, Yasuyuki
在实体瘤中，癌细胞暴露于微环境应激，包括缺氧和营养不足。乳酸合成的增加促进了肿瘤中的酸性微环境;这被称为瓦尔堡效应。我们以前的研究表明，B16 F10黑色素瘤细胞由葡萄糖剥夺应激诱导自噬性细胞死亡，乳酸通过抑制自噬抑制细胞死亡。本研究探讨了乳酸对缺氧缺糖双重应激条件下B16 F10细胞死亡的影响。双重压力促进自噬细胞死亡早于葡萄糖耗尽的压力。乳酸通过抑制自噬抑制双重应激诱导的细胞死亡。这些结果表明，乳酸在B16 F10黑色素瘤细胞中的微环境应激条件下对细胞存活起作用。
In solid tumors, cancer cells are exposed to microenvironment stress, including hypoxia and insufficient nutrients. An acidic microenvironment in tumors is facilitated by the increase in synthesis of lactic acid; this is known as Warburg effect. We previously showed that B16F10 melanoma cells were induced autophagic cell death by glucose-deprivation stress, and lactic acid suppressed the cell death through the inhibition of autophagy. In this study, effects of lactic acid on cell death of B16F10 cells under hypoxic and glucose-depleted double stress conditions were investigated. The double stress promoted autophagic cell death earlier than glucose-depleted stress alone. Lactic acid repressed the double stress-induced cell death by inhibiting autophagy. These results suggest that lactic acid serves for cell survival under microenvironmental stress conditions in B16F10 melanoma cells.