The propeptide of rat bone gamma-carboxyglutamic acid protein shares homology with other vitamin K-dependent protein precursors.
The propeptide of rat bone gamma-carboxyglutamic acid protein shares homology with other vitamin K-dependent protein precursors.
复制标题
大鼠骨γ-羧基谷氨酸蛋白的前肽与其他维生素K依赖性蛋白前体具有同源性。
DOI：
10.1073/pnas.82.18.6109
复制
发表时间：
1985
影响因子：
11.1
通讯作者：
Price,PA
中科院分区：
文献类型：
--
作者：
Pan,LC;Price,PA
通过构建大鼠骨肉瘤细胞系ROS 17/2的噬菌体λ gt 11 cDNA文库，并用从大鼠骨中产生的抗骨钙素（BGP）的抗体筛选该文库，完成了骨钙素（BGP）的分子克隆。通过对几个克隆的cDNA进行测序，我们已经建立了一个489个碱基对的序列，该序列预测了50个氨基酸残基的成熟BGP，其中NH 2末端延伸49个残基。前导肽由疏水信号肽和26或27个残基的碱性前肽组成，所述碱性前肽在Arg-Arg二肽之后裂解，然后从细胞中分泌。成熟的大鼠BGP与其他物种的BGP除了其COOH末端序列外具有高度的同源性。一段9个残基近端的NH 2末端的分泌BGP是惊人的相似，在已知的前肽的γ-羧基谷氨酸含凝血因子的相应区域。我们认为，这一共同的结构特征可能涉及这些多肽的翻译后靶向维生素K依赖性γ-羧化。
The molecular cloning of bone gamma-carboxyglutamic acid (Gla) protein (BGP; osteocalcin) was accomplished by constructing a phage lambda gt11 cDNA library from the rat osteosarcoma cell line ROS 17/2 and screening this library with antibodies raised against BGP from rat bone. By sequencing several cloned cDNAs, we have established a 489-base-pair sequence that predicts a mature BGP of 50 amino acid residues with an NH2-terminal extension of 49 residues. The leader peptide consists of a hydrophobic signal peptide followed by a basic propeptide of 26 or 27 residues that is cleaved after an Arg-Arg dipeptide prior to secretion from the cell. Mature rat BGP is extremely homologous to BGPs from other species except for its COOH-terminal sequence. A stretch of 9 residues proximal to the NH2 terminus of secreted BGP is strikingly similar to the corresponding regions in known propeptides of the gamma-carboxyglutamic acid-containing blood coagulation factors. We suggest that this common structural feature may be involved in the posttranslational targeting of these polypeptides for vitamin K-dependent gamma-carboxylation.