The effect of pyruvate decarboxylase gene knockout in Saccharomyces cerevisiae on L-lactic acid production
The effect of pyruvate decarboxylase gene knockout in Saccharomyces cerevisiae on L-lactic acid production
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DOI：
10.1271/bbb.70.1148
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发表时间：
2006-05-01
影响因子：
1.6
通讯作者：
Takahashi, Haruo
中科院分区：
文献类型：
--
作者：
Ishida, Nobuhiro;Saitoh, Satoshi;Takahashi, Haruo
以植物和作物为基础的可再生塑料聚乳酸（PLA）作为一种替代石油基塑料的可持续社会新材料正受到关注。我们构建了一株代谢工程的酿酒酵母，该酵母的两个丙酮酸脱氢酶基因（PDC1和PDC5）在遗传背景中被破坏，以表达两个拷贝的牛l -乳酸脱氢酶（LDH）基因。重组后的乳酸产率为82.3g/l，中和培养时葡萄糖转化为乳酸的比例高达81.5%，但pdc1 pdc5的双重破坏导致细胞生长和发酵速度的无效降低。该菌株乳酸产量比前一菌株提高了1.5倍。这一产量是迄今为止报道的乳酸生产酵母的最高值。
A plant- and crop-based renewable plastic, poly-lactic acid (PLA), is receiving attention as a new material for a sustainable society in place of petroleum-based plastics. We constructed a metabolically engineered Saccharomyces cerevisiae that has both pyruvate decarboxylase genes (PDC1 and PDC5) disrupted in the genetic background to express two copies of the bovine L-lactate dehydrogenase (LDH) gene. With this recombinant, the yield of lactate was 82.3g/liter, up to 81.5% of the glucose being transformed into lactic acid on neutralizing cultivation, although pdc1 pdc5 double disruption led to ineffective decreases in cell growth and fermentation speed. This strain showed lactate productivity improvement as much as 1.5 times higher than the previous strain. This production yield is the highest value for a lactic acid-producing yeast yet reported.