High CCR7 mRNA expression of cancer cells is associated with lymph node involvement in patients with esophageal squamous cell carcinoma
High CCR7 mRNA expression of cancer cells is associated with lymph node involvement in patients with esophageal squamous cell carcinoma
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DOI：
10.3892/ijo_00000217
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发表时间：
2009-04-01
影响因子：
5.2
通讯作者：
Yamaue, Hiroki
中科院分区：
文献类型：
--
作者：
Ishida, Koichiro;Iwahashi, Makoto;Yamaue, Hiroki
食管鳞状细胞癌是一种生物学恶性肿瘤。一旦肿瘤侵入粘膜下层，淋巴结（LN）转移的发生率非常高，从而导致较差的存活率。最近，趋化因子已被报道在几种恶性肿瘤的器官特异性转移中发挥重要作用。特别是，CCR 7已被报道与LN转移免疫组织化学。然而，还没有研究定量分析CCR 7 mRNA在癌细胞上的表达。本研究旨在探讨CCR 7在食管鳞癌LN转移中的表达及其临床意义。连续选择了78例接受食管切除术的食管鳞癌患者。采用实时定量逆转录聚合酶链反应（qRT-PCR）分析肿瘤组织标本中CCR 7 mRNA的表达，采用激光显微切割系统收集癌细胞标本，采用qRT-PCR分析CCR 7 mRNA的表达。还进行了CCR 7的免疫组织化学染色。尽管肿瘤组织中CCR 7 mRNA的表达与淋巴结转移无关，但癌细胞中CCR 7 mRNA的表达与淋巴结转移相关（p
Esophageal squamous cell carcinoma (SCC) is one of the biological malignant tumors. Once a tumor invades the submucosa, an incidence of lymph node (LN) metastases is very high, thus resulting in poor survival. Recently, chemokines have been reported to play an important role in organ-specific metastases in several malignancies. In particular, CCR7 has been reported to be associated with LN metastases by immunohistochemistry. However, there have been no studies of quantitative analyses of CCR7 mRNA expression on cancer cells. In this study, we investigated the clinical significance of the expression of CCR7 in the establishment of LN metastases of esophageal SCC. A series of 78 patients with esophageal SCC who underwent esophagectomy were consecutively selected. The expression of CCR7 mRNA from tumor tissue samples was analyzed by quantitative real-time reverse transcriptase-polymerase chain reaction (qRT-PCR), and that from cancer cell samples collected using laser microdissection system was analyzed by qRT-PCR. Immunohistochemical staining of CCR7 was also performed. Although CCR7 mRNA expression in tumor tissues demonstrated no association with the LN metastases, that in cancer cells correlated with LN metastases (p