Electrochemically Triggered Selective Adsorption of Biotemplated Nanoparticles on Self-Assembled Organometallic Diblock Copolymer Thin Films
Electrochemically Triggered Selective Adsorption of Biotemplated Nanoparticles on Self-Assembled Organometallic Diblock Copolymer Thin Films
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DOI：
10.1002/adfm.201200210
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发表时间：
2012-08-07
影响因子：
19
通讯作者：
Schwarzacher, Walther
中科院分区：
文献类型：
--
作者：
Eloi, Jean-Charles;Jones, Sarah E. Ward;Schwarzacher, Walther
报道了利用刺激响应性自组装二嵌段共聚物薄膜模板在纳米尺度上对含铁笼蛋白铁蛋白的可控吸附。研究所用的二嵌段共聚物由圆柱形聚苯乙烯-嵌段-聚二茂铁基硅烷（PS-b-PFS）组成，PFS为次嵌段，并显示可逆的氧化还原性能。为了防止任何自发的蛋白质吸附在任一块上，选择电解质pH以使铁蛋白带负电荷，并仔细调节蛋白质浓度和溶液离子强度。然后通过施加正电势原位触发铁蛋白在自组装薄膜的PFS域上的选择性吸附，同时氧化PFS并静电吸引铁蛋白。
The controlled adsorption of the iron-containing cage protein ferritin at the nanoscale using stimuli-responsive self-assembled diblock copolymer thin-film templates is reported. The diblock copolymer used study consists of a cylinder-forming polystyrene-block-polyferrocenylsilane (PS-b-PFS), with PFS as the minor block, and shows reversible redox properties. To prevent any spontaneous protein adsorption on either block, the electrolyte pH is selected to leave the ferritin negatively charged, and the protein concentration and solution ionic strength are carefully tuned. Selective adsorption of ferritin on the PFS domains of the self-assembled thin films is then triggered in situ by applying a positive potential, simultaneously oxidizing the PFS and attracting the ferritin electrostatically.