LOCALIZATION OF 5-HT1B, 5-HT1D-ALPHA, 5-HT1E AND 5-HT1F, RECEPTOR MESSENGER-RNA IN RODENT AND PRIMATE BRAIN
LOCALIZATION OF 5-HT1B, 5-HT1D-ALPHA, 5-HT1E AND 5-HT1F, RECEPTOR MESSENGER-RNA IN RODENT AND PRIMATE BRAIN
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DOI：
10.1016/0028-3908(94)90067-1
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发表时间：
1994-03-01
影响因子：
4.7
通讯作者：
PALACIOS, JM
中科院分区：
文献类型：
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作者：
BRUINVELS, AT;LANDWEHRMEYER, B;PALACIOS, JM
应用原位杂交组织化学（ISHH）技术研究了5-HT_1受体信使RNA（mRNA）在哺乳动物神经系统中的分布。由于编码不同5-HT 1受体的cDNA尚未在单一物种中克隆，因此已使用适合于5-HT 1受体信使RNA（mRNA）的物种的脑。因此，5-HT 1B和5-HT 1D α mRNA在大鼠和小鼠的大脑中进行了测定，而5-HT 1 E和5-HT 1F mRNA在人类（和猴子）和豚鼠的大脑中进行了研究，分别。5-HT 1B和5-HT 1D α杂交信号主要存在于尾壳核和皮质区;此外，5-HT 1B mRNA也在海马，小脑和脑动脉中检测到。一般来说，5-HT 1B mRNA的分布特点是高密度，而5-HT 1D α mRNA的表达水平很低。大鼠脑内5-HT 1B和5-HT 1D结合位点的区域分布与mRNA定位的比较（在先前的研究中描述），揭示了这两种受体亚型可以是推定的突触前异源受体，调节中枢神经系统中各种神经递质的释放。其对5-HT 1受体配体5-羧酰胺色胺（5-CT）具有低亲和力。它被发现存在于皮质区，尾状核，壳核和杏仁核，已知含有5-CT不敏感的5-HT 1结合位点的区域。在豚鼠脑中测定5-HT 1F mRNA的区域分布：在不同的皮质区域、海马结构和屏状核中观察到高密度，这些区域已知含有5-CT不敏感的5-HT 1或非5-HT 1A/1B/1C/1D [H-3]5-HT结合位点。这项ISHH研究描述了最近克隆的5-HT 1受体的mRNA在啮齿动物和灵长类动物脑中的分布，并将这些结果与5-HT 1结合位点的异质群体的分布进行了比较。
In situ hybridization histochemistry (ISHH) was used to study the distribution of various 5-HT1 receptor messenger RNAs (mRNA) in the mammalian nervous system. Since the cDNAs encoding the different 5-HT1 receptors, have not been cloned in one single species, brains of the species appropriate for the 5-HT1 receptor messenger RNA (mRNA) have been used. Thus, 5-HT1B and 5-HT1D alpha mRNA were determined in rat and mouse brain, while 5-HT1E and 5-HT1F mRNA were studied in human (and monkey) and guinea-pig brain, respectively.5-HT1B and 5-HT1D alpha hybridization signals were predominantly present in caudate-putamen and cortical areas; in addition, 5-HT1B mRNA was also detected in hippocampus, cerebellum and cerebral arteries. In general, the distribution of 5-HT1B mRNA was characterized by high densities, whereas 5-HT1D alpha mRNA was expressed at very low levels. Comparison of the localization of the mRNAs to the regional distributions of the 5-HT1B and 5-HT1D binding sites in rat brain (described in a previous study), revealed that both receptor subtypes could be putative presynaptic heteroreceptors, modulating the release of various neurotransmitters in the central nervous system.The mRNA encoding the recently cloned 5-HT1E receptor, which has low affinity for the 5-HT1 receptor ligand 5-carboxamidotryptamine (5-CT), was localized in human brain. It was found to be present in cortical areas, caudate, putamen and amygdala, areas known to contain 5-CT insensitive 5-HT1 binding sites. The regional distribution of the 5-HT1F mRNA was determined in guinea-pig brain: high densities were observed in various cortical areas, the hippocampal formation and claustrum, which are regions known to contain 5-CT insensitive 5-HT1, or non 5-HT1A/1B/1C/1D [H-3]5-HT binding sites.Altogether, this ISHH study describes the distribution of mRNAs of recently cloned 5-HT1 receptors in rodent and primate brain and compares these results to the distribution of the heterogeneous population of 5-HT1 binding sites.