Bmcas-1 plays an important role in response against BmNPV infection in vitro
Bmcas-1 plays an important role in response against BmNPV infection in vitro
复制标题
Bmcas-1 在体外对抗 BmNPV 感染的反应中发挥重要作用
DOI：
10.1002/arch.21793
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发表时间：
2021
影响因子：
2.2
通讯作者：
Wang Xue-yang
中科院分区：
文献类型：
--
作者：
Wang Xin;Zhao Zi-qin;Huang Xin-ming;Ding Xin-yi;Zhao Chun-xiao;Li Mu-wang;Wu Yang-chun;Liu Qiu-ning;Wang Xue-yang
细胞凋亡作为一种天然免疫系统，参与宿主对病原体的创新反应。Caspase在宿主细胞凋亡的执行阶段起着重要作用。据报道，Bmcaspase-1（Bmcas-1）与家蚕核多角体病毒（BmNPV）感染关系密切，其在病毒感染后的表达模式存在差异。然而，其潜在的反应机制仍不清楚。Bmcas-1在不同抗性株不同组织中的差异表达揭示了其在BmNPV感染中的重要作用。为了进一步验证其在BmNPV感染中的作用，分别通过小干扰RNA和pIZT-mCherry载体敲低和过表达Bmcas-1后，分析了出芽病毒（BV）-eGFP。Bmcas-1基因敲除后72 h，BV-eGFP的表达量明显增加，而BmN细胞中BV-eGFP的表达量明显降低。此外，Cas-1在不同物种中保守的功能结构域以及Cas-1在鳞翅目中的紧密进化关系暗示Bmcas-1可能与细胞凋亡相关，这也被细胞凋亡诱导剂Silvestrol和抑制剂Z-DEVD-FMK所证实。因此，Bmcas-1通过激活细胞凋亡发挥重要的抗病毒作用，这一结果为阐明家蚕抗BmNPV感染的分子机制和抗病品系的选育奠定了基础。
Apoptosis, as one kind of innate immune system, is involved in host response against pathogens innovation. Caspases play a vital role in the execution stage of host cell apoptosis. It has been reported that Bmcaspase‐1 (Bmcas‐1) has a close relationship withBombyx morinucleopolyhedrovirus (BmNPV) infection for its differentially expressed patterns after viral infection. However, its underlying response mechanism is still unclear. The significant differential expression ofBmcas‐1in different tissues of differentially resistant strains revealed its vital role in BmNPV infection. To further validate its role in BmNPV infection, budded virus (BV)‐eGFP was analyzed after knockdown and overexpression ofBmcas‐1by small interfering RNA and the pIZT‐mCherry vector, respectively. The reproduction of BV‐eGFP obviously increased at 72 h after knockdown ofBmcas‐1, and decreased after overexpression in BmN cells. Moreover, the conserved functional domain of Cas‐1 among different species and the closed evolutionary relationship of Cas‐1 in Lepidoptera hinted that Bmcas‐1 might be associated with apoptosis, and this was also validated by the apoptosis inducer, Silvestrol, and the inhibitor, Z‐DEVD‐FMK. Therefore,Bmcas‐1plays an essential antiviral role by activating apoptosis, and this result lays a fundament for clarifying the molecular mechanism of silkworm in response against BmNPV infection and breeding of resistant strains.