Association of CLU gene polymorphism with Parkinson'sdisease in the Chinese Han population
Association of CLU gene polymorphism with Parkinson'sdisease in the Chinese Han population
复制标题
中国汉族人群CLU基因多态性与帕金森病的关联
DOI：
10.1002/jgm.3302
复制
发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Pingyi Xu
中科院分区：
文献类型：
--
作者：
Yuwan Lin;Lin Lu;Miaomiao Zhou;HanQun Liu;Panghai Ye;Wenlong Zhang;Jiewen Qiu;Zhiling Zhang;Xinling Yang;Liuyan Ding;Wenyuan Guo;Mingshu Mo;Xiaoqin Zhu;Xiaokang Zhang;Xiang Chen;Pingyi Xu
背景：Clusterin （CLU）在神经退行性疾病的病理中起重要作用。最近，CLU rs9331896的一种遗传变异被报道为阿尔茨海默病（AD）的风险评估。然而，这之间的联系。中国汉族人群帕金森病（PD）的变异和风险。方法：我们对353名PD患者和326名健康匹配的中国汉族人群的CLU rs9331896进行了测序。rs9331896的基因型。使用MassArray （Agena Bioscience, San Diego， CA， USA）按照制造商说明进行分析。基因型和等位基因频率分布采用卡方检验。此外，CLU的表达。血浆蛋白用酶联免疫吸附法测定。用t检验分析…结果：隐性模型rs9331896中TT基因型与PD发病风险增加相关（优势比= 1.408,95%置信区间= 1.034 ~ 1.916,p = 0.029）。亚组分析显示TT基因型携带者在男性PD患者中的风险明显高于健康男性。对照组（优势比= 1.611,95%可信区间= 1.046-2.483,p = 0.030）。在。PD患者血浆CLU水平显著高于对照组（p = 0.024）。结论：CLU-rs9331896-TT基因型是帕金森病的危险因素。在男性。PD患者在血浆中也表达高水平的CLU。
Background: Clusterin (CLU) plays important role in the pathology of neurodegenerative disorders. Recently, a genetic variant of CLU rs9331896 has been reported as a.risk estimate for Alzheimer’s disease (AD). However, the association between this.variant and the risk of Parkinson's disease (PD) in the Chinese Han population.remains elusive..Methods: We sequenced CLU rs9331896 in 353 PD patients and 326 healthymatched individuals of the Chinese Han population. The genotypes of rs9331896.were analyzed using MassArray (Agena Bioscience, San Diego, CA, USA) in accordance with the manufacturer’s instructions. The distribution of genotypes and allelic.frequencies was analyzed by a chi-squared test. Additionally, the expression of CLU.protein in plasma was evaluated by an enzyme-linked immunosorbent assay and.analysed with a t-test..Results: The TT genotype in rs9331896 in a recessive model was found to be associated with the increased risk of PD (odds ratio = 1.408, 95% confidence.interval = 1.034–1.916, p = 0.029). Subgroup analysis indicated that TT genotype carriers showed a significantly higher risk in male PD patients compared to male healthy.controls (odds ratio = 1.611, 95% confidence interval = 1.046–2.483, p = 0.030). In.addition, CLU levels in the plasma of PD patients were significantly higher than controls (p = 0.024)..Conclusions: The CLU-rs9331896-TT genotype was a risk factor for PD, particularly.in males. PD patients also expressed a high level of CLU in plasma.