Non-reciprocal cross-adaptation of spiking responses of individual olfactory receptor neurons of spiny lobsters: evidence for two excitatory transduction pathways.
Non-reciprocal cross-adaptation of spiking responses of individual olfactory receptor neurons of spiny lobsters: evidence for two excitatory transduction pathways.
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大龙虾个体嗅觉受体神经元尖峰反应的非相互交叉适应：两种兴奋性转导途径的证据。
DOI：
10.1016/0006-8993(94)90019-1
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发表时间：
1994
期刊：
影响因子：
2.9
通讯作者：
Girardot,MN
中科院分区：
文献类型：
--
作者：
Daniel,PC;Fine,JB;Derby,CD;Girardot,MN
在细胞外记录了72个多刺龙虾嗅器嗅觉受体神经元（ORN）对一组7种气味刺激（5′-磷酸腺苷、氯化铵、甜菜碱、l-半胱氨酸、l-谷氨酸、d，l-琥珀酸和牛磺酸）的单单位放电反应，并分析了单个ORN的反应特异性和受体位点的独立性。个别ORNs往往有狭窄的兴奋性反应谱，但最兴奋的化合物是不同的神经元。这些结果表明，这些化合物可以发挥其兴奋作用，通过相对独立的受体位点类型。为了确定这些刺激在单个ORN中的兴奋性转导过程的相对独立性，分析了这些神经元在自适应和交叉适应条件下的单单位尖峰响应。结果表明，广泛的交叉适应之间的七个刺激对。当在所有神经元和所有交叉适应条件下取平均值时，交叉适应导致非适应反应平均减少81%。然而，有不同的化合物对和不同的神经元类型（定义为最兴奋或“最好的”化学）的适应程度和模式的差异。对于一个给定的神经元类型，有显着水平的非互惠交叉适应：神经元交叉适应时，适应他们最好的化学品比适应他们的非最好的化学品。这些结果表明嗅觉受体神经元内存在两种兴奋性转导途径：一种途径专门由最好的化学物质激活，第二种途径由更广泛的化学物质激活。
Single-unit spiking responses of 72 olfactory receptor neurons (ORNs) in the olfactory organ of the spiny lobsterPanulirus arguswere recorded extracellularly during presentation of a set of seven odorant stimuli (adenosine-5′-monophosphate, ammonium chloride, betaine,l-cysteine,l-glutamate,d,l-succinate and taurine) and analyzed in order to evaluate the response specificities of single ORNs and the independence of receptor sites. Individual ORNs often had narrow excitatory response spectra, but the most excitatory compound was different from neuron to neuron. These results suggest that these compounds can exert most of their excitatory effects through relatively independent receptor site types. To determine the relative independence of excitatory transduction processes in single ORNs for these stimuli, single-unit spiking responses of these neurons under conditions of self- and cross-adaptation were analyzed. The results demonstrate extensive cross-adaptation between pairs of the seven stimuli. When averaged across all neurons and all cross-adaptation conditions, cross-adaptation resulted in a mean reduction of 81% of the unadapted response. However, there were differences in the degree and pattern of adaptation for different pairs of compounds and for different neuron types (defined by most excitatory or ‘best’ chemical). For a given neuron type, there were significant levels of non-reciprocal cross-adaptation: neurons cross-adapted more when adapted to their best chemical than when adapted to their non-best chemicals. These results suggest the existence of two excitatory transduction pathways within an olfactory receptor neuron: one pathway activated exclusively by the best chemical and a second pathway activated by a broader spectrum of chemicals.