A versatile and highly sensitive probe for Hg(II), Pb(II) and Cd(II) detection individually and totally in water samples
A versatile and highly sensitive probe for Hg(II), Pb(II) and Cd(II) detection individually and totally in water samples
复制标题
多功能、高灵敏度探头，可单独和全面检测水样中的 Hg(II)、Pb(II) 和 Cd(II)
DOI：
10.1016/j.bios.2011.08.034
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发表时间：
2011-12-15
影响因子：
12.6
通讯作者：
Liu, Zeng-Shan
中科院分区：
文献类型：
--
作者：
Zhou, Yu;Tian, Xiang-Li;Liu, Zeng-Shan
几个研究小组已经报道了使用酶联免疫吸附测定（ELISA）检测重金属离子。然而，高灵敏度和选择性检测的总重金属离子使用ELISA是一个主要的技术限制。在这里，我们描述了一个通用的和高灵敏度的探针结合山羊抗小鼠IgG，胶体金纳米粒子（AuNPs）和辣根过氧化物酶（HRP）的发展。我们使用三种重金属完全抗原和三种单克隆抗体（McAb）在一个ELISA系统中证明了该探针的实用性，以建立高通量筛选方案。该方法已成功地应用于汞（II），铅（II）和镉（II）单独和全部从不同的水样的分析。测定Hg（II）的灵敏度。Pb（II）和Cd（II）的浓度分别为27.4、3.9、15.8和18.2 nM。检测限（LOD）均低于1.2nM。该方法的回收率与ICP-MS的回收率具有良好的相关性（R-2 = 0.983）。该探头可以潜在地使用，根据需求，作为一个敏感的和多功能的检测器，用于广泛的应用。(C)2011爱思唯尔有限公司版权所有。
The detection of heavy metal ions using enzyme-linked immunosorbent assays (ELISA) has been reported by several research groups. However, highly sensitive and selective detection of total heavy metal ions using ELISA is a major technical limitation. Here we describe the development of a versatile and highly sensitive probe combining goat anti-mice IgG, colloidal gold nanoparticles (AuNPs) and horseradish peroxidase (HRP). We demonstrate the utility of this probe using three kinds of heavy metal complete antigens and three monoclonal antibodies (McAbs) in one ELISA system to establish a high-throughput screening protocol. The procedure was successfully applied to analysis of Hg(II), Pb(II) and Cd(II) individually and totally from different water samples. The sensitivities for the detection of Hg(II). Pb(II) and Cd(II) individually and totally are 27.4, 3.9, 15.8 and 18.2 nM, respectively. And all limit of detection (LODs) are lower than 1.2 nM. The recovery results obtained from the developed technique showed a good correlation (R-2 = 0.983) with those from ICP-MS. The major advantage of the probe is the versatility and high sensibility. The probe could be potentially used, upon demand, as a sensitive and versatile detector for a broad range of applications. (C) 2011 Elsevier B.V. All rights reserved.