Concentrations of tryptoline and methtryptoline in rat brain.
Concentrations of tryptoline and methtryptoline in rat brain.
复制标题
大鼠脑中色氨酸和甲基色氨酸的浓度。
DOI：
10.1111/j.1471-4159.1989.tb02531.x
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发表时间：
1989
影响因子：
4.7
通讯作者：
Faull,KF
中科院分区：
文献类型：
--
作者：
Peura,P;Johnson,JV;Yost,RA;Faull,KF
采用气相色谱-质谱法和气相色谱-串联质谱法相结合的方法，对大鼠脑提取液中的七氟丁酰基衍生物类化合物--色氨酸、甲基色氨酸、5-羟基-5-甲基-色氨酸进行了鉴定和定量。色氨酸和甲基色氨酸是根据保留时间和质谱学特征确定的：它们在脑组织提取物中可靠地检测到，不受人工形成的干扰；它们在整个大脑中的浓度在0.2到3 ng/g之间；它们具有相似的神经解剖分布，在小脑和皮质中浓度最高。根据保留时间和质谱学特征，还鉴定了少量的5-羟基类和5-羟甲基类。然而，这一发现的意义尚不清楚，因为这两种化合物在组织匀浆时都伴随着更多的四氢类似物，这些类似物是在组织匀浆时加入的四氢-5-羟色胺形成的；这一结果表明，5-羟色胺和5-羟甲基色胺的形成发生在组织匀浆或样品制备过程中，或者两者兼而有之。
Combined gas chromatography‐mass spectrometry and gas chromatography‐tandem mass spectrometry have been used to identify and quantify tryptoline, methtryptoline, 5‐hydroxytryptoline, and 5‐hydroxymethtryptoline as their heptafluorobutyryl derivatives in extracts of rat brain. Tryptoline and methtryptohne were identified on the basis of their retention times and mass spectral characteristics: they were reliably detected in brain tissue extracts without interference from artifactual formation; their whole brain concentrations ranged between 0.2 and 3 ng/g; and they had a similar neu‐roanatomical distribution, with the highest concentrations in the cerebellum and the cortex. Smaller quantities of 5‐hydroxytryptoline and 5‐hydroxymethtryptoline were also identified on the basis of their retention times and mass spectral characteristics. However, the significance of this finding is unclear, because these two compounds were accompanied by larger quantities of their tetradeuterated analogues formed from tetradeuterated‐5‐hydroxytryptamine added at the time of tissue homogenization; this result suggests that formation of 5‐hydroxytryptoline and 5‐hydroxymethtryptoline occurred during tissue homogenization, sample preparation, or both.