Detection of target ssDNA using a microfabricated Hall magnetometer with correlated optical readout.
Detection of target ssDNA using a microfabricated Hall magnetometer with correlated optical readout.
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使用具有相关光学读数的微型霍尔磁力计检测目标 ssDNA。
DOI：
10.1155/2012/492730
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发表时间：
2012
影响因子：
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通讯作者：
Strouse,GeoffreyF
中科院分区：
文献类型：
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作者：
Hira,StevenM;Aledealat,Khaled;Chen,Kan-Sheng;Field,Mark;Sullivan,GerardJ;Chase,PBryant;Xiong,Peng;vonMolnar,Stephan;Strouse,GeoffreyF
在基因组水平上感测生物制剂，同时增强生物检测的响应时间，超过常用的基于光学的技术，如核酸微阵列或酶联免疫吸附测定（ELISA），是新型生物传感器的重要标准。在这里，我们描述了通过基于霍尔的磁转导成功检测35个碱基的单链核酸靶标，作为病原性DNA靶标检测的模拟物。该检测平台具有低背景、靶结合后的大信号放大，并且可以区分用DNA标记的单个350 nm超顺磁珠。在存在36μM非靶（非互补）DNA（<10 ppm靶DNA）的情况下，使用GaAs Hall模拟物上的光学显微镜检测，在364 pM（每个微珠<2条靶DNA链）靶DNA下证明了靶序列的检测。使用霍尔磁力计作为磁转导生物传感器为多路复用应用带来了希望，这些应用可以大大改善床旁（POC）诊断和后续医疗护理。
Sensing biological agents at the genomic level, while enhancing the response time for biodetection over commonly used, optics‐based techniques such as nucleic acid microarrays or enzyme‐linked immunosorbent assays (ELISAs), is an important criterion for new biosensors. Here, we describe the successful detection of a 35‐base, single‐strand nucleic acid target by Hall‐based magnetic transduction as a mimic for pathogenic DNA target detection. The detection platform has low background, large signal amplification following target binding and can discriminate a single, 350 nm superparamagnetic bead labeled with DNA. Detection of the target sequence was demonstrated at 364 pM (<2 target DNA strands per bead) target DNA in the presence of 36μM nontarget (noncomplementary) DNA (<10 ppm target DNA) using optical microscopy detection on a GaAs Hall mimic. The use of Hall magnetometers as magnetic transduction biosensors holds promise for multiplexing applications that can greatly improve point‐of‐care (POC) diagnostics and subsequent medical care.