Epidermal growth factor receptor mRNA and protein increase after the four-cell preimplantation stage in murine development.
Epidermal growth factor receptor mRNA and protein increase after the four-cell preimplantation stage in murine development.
复制标题
小鼠发育中四细胞植入前阶段后表皮生长因子受体 mRNA 和蛋白质增加。
DOI：
10.1016/0012-1606(92)90282-l
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发表时间：
1992
影响因子：
2.7
通讯作者：
Adamson,ED
中科院分区：
文献类型：
--
作者：
Wiley,LM;Wu,JX;Harari,I;Adamson,ED
表皮生长因子受体(EGF-Rs)在小鼠着床前胚胎上的表达水平越来越高。免疫荧光分析表明，未受精卵和2-细胞胚胎对抗EGF-R抗体的反应性很低，但在4-细胞期和以后，反应性增强。在胚泡阶段，通过免疫沉淀170 kDa的代谢性标记蛋白证实了EGF-R蛋白的合成。EGF-R蛋白在细胞质膜表面表达，但在8-细胞期压实后集中在顶端细胞表面。我们还发现EGF-R蛋白在内细胞团细胞上的表达水平很低，因此，所有细胞系从妊娠开始就表达受体。由8-细胞胚胎合成的受体蛋白可能是从胚胎转录而来的，因为逆转录-聚合酶链式反应表明，在4-细胞阶段之后，mRNA的水平开始增加。然而，我们也在受精卵和2-细胞胚胎中检测到母体mRNA。EGF-R在发育过程中的普遍表达暗示了这些受体的重要作用，其中可能包括自分泌和旁分泌刺激。
Epidermal growth factor receptors (EGF-Rs) are expressed at increasing levels on mouse preimplantation embryos. Immunofluorescence assays were used to show that unfertilized eggs and 2-cell embryos have a very low level of reactivity to antimouse EGF-R antibodies, but by the 4-cell stage and later the reactivity increases. The synthesis of EGF-R protein was verified at the blastocyst stage by immunoprecipitation of a 170-kDa metabolically labeled protein. The EGF-R protein is expressed on cell plasma membrane surfaces, but after compaction at the 8-cell stage concentrates on the apical cell surfaces. We also find a low level of expression of EGF-R protein on inner cell mass cells; thus, all cell lineages express receptors from the beginning of gestation. The receptor protein synthesized by the 8-cell embryo and later is probably translated from embryonic transcription, since reverse transcription-polymerase chain reaction indicates increasing levels of mRNA starting after the 4-cell stage. However, we also detected maternal mRNA in zygotes and 2-cell embryos. The pervasive nature of EGF-R expression throughout development suggests important roles for these receptors which could include autocrine and paracrine stimulation.