Sex drives colonic mucin sialylation in wild mice
Sex drives colonic mucin sialylation in wild mice
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DOI：
10.1038/s41598-024-57249-x
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发表时间：
2024-03-23
影响因子：
4.6
通讯作者：
Thornton，David J.
中科院分区：
文献类型：
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作者：
Bennett，Alexander R.;Mair，Iris;Thornton，David J.
粘蛋白糖基化在确定粘液凝胶的生物学特性方面很重要，粘液凝胶在人体的粘膜表面形成保护性屏障，如肠道。包括年龄、性别和饮食在内的生态因素可以通过调节粘蛋白糖基化来改变粘液屏障特性。然而，由于我们的理解源于对家鼠的对照实验室研究，在现实世界中，生态因素对粘蛋白糖基化的联合影响仍然有限。在这项研究中，我们使用‘阿尔新蓝，高碘酸，希夫氏’和‘高铁二胺’的组织学染色来评估储存在肠道杯状细胞内的粘蛋白的酸性性质，在一个野生老鼠种群(Mus Musculus)中。利用统计模型，我们确定性别是决定野生小鼠肠道粘蛋白多糖酸度的最有影响的生态因素之一。我们来自野生老鼠的数据和使用实验室老鼠的实验表明，雌激素信号与唾液酸化粘蛋白的相对丰度增加有关。因此，雌激素信号可能是野生小鼠和实验室小鼠结肠粘液观察到的性别差异的基础。这些发现突出了生态参数对粘膜屏障位置的显著影响，以及野生种群在加强粘液生物学进步中的标准实验室研究方面的补充作用。
Mucin protein glycosylation is important in determining biological properties of mucus gels, which form protective barriers at mucosal surfaces of the body such as the intestine. Ecological factors including: age, sex, and diet can change mucus barrier properties by modulating mucin glycosylation. However, as our understanding stems from controlled laboratory studies in house mice, the combined influence of ecological factors on mucin glycosylation in real-world contexts remains limited. In this study, we used histological staining with ‘Alcian Blue, Periodic Acid, Schiff’s’ and ‘High-Iron diamine’ to assess the acidic nature of mucins stored within goblet cells of the intestine, in a wild mouse population (Mus musculus). Using statistical models, we identified sex as among the most influential ecological factors determining the acidity of intestinal mucin glycans in wild mice. Our data from wild mice and experiments using laboratory mice suggest estrogen signalling associates with an increase in the relative abundance of sialylated mucins. Thus, estrogen signalling may underpin sex differences observed in the colonic mucus of wild and laboratory mice. These findings highlight the significant influence of ecological parameters on mucosal barrier sites and the complementary role of wild populations in augmenting standard laboratory studies in the advancement of mucus biology.