The chemical composition of synthetic bone substitutes influences tissue reactions in vivo: histological and histomorphometrical analysis of the cellular inflammatory response to hydroxyapatite, beta-tricalcium phosphate and biphasic calcium phosphate ceramics
The chemical composition of synthetic bone substitutes influences tissue reactions in vivo: histological and histomorphometrical analysis of the cellular inflammatory response to hydroxyapatite, beta-tricalcium phosphate and biphasic calcium phosphate ceramics
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DOI：
10.1088/1748-6041/7/1/015005
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发表时间：
2012-02-01
影响因子：
4
通讯作者：
Kirkpatrick, Charles James
中科院分区：
文献类型：
--
作者：
Ghanaati, Shahram;Barbeck, Mike;Kirkpatrick, Charles James
骨替代材料的性质，如颗粒大小、大孔隙率、微孔率和形状，已被证明会影响对骨替代材料的细胞炎症反应。保持这些参数不变，本研究分析了体内组织对三种不同化学成分（羟基磷灰石（HA）、β-磷酸三钙（TCP）以及HA/TCP比例为60/40 wt %的混合物）的骨替代材料（颗粒）的反应。使用Wistar大鼠皮下植入模型长达30天，使用组织学和组织形态计量学分析评估组织反应，包括多核巨细胞的诱导和植入床血管化的程度。结果表明，骨替代材料的化学成分显著影响细胞反应。与HA相比，TCP在植入床内吸引了更多的多核巨细胞形成，而且TCP植入床的血管化程度明显高于HA植入床。双相骨替代物组结合了两组的特性。在前15天内，观察到高巨细胞形成和血管化率，与TCP组相当。然而，15天后，组织反应，即多核巨细胞形成和血管化的程度，与HA组相当。总之，化合物HA和TCP的组合可以是用于产生支架的有用组合，用于在植入后的早期时间点期间快速血管化和整合，以及用于建立相对缓慢的降解。这两个因素都是成功的骨组织再生所必需的。
Bone substitute material properties such as granule size, macroporosity, microporosity and shape have been shown to influence the cellular inflammatory response to a bone substitute material. Keeping these parameters constant, the present study analyzed the in vivo tissue reaction to three bone substitute materials (granules) with different chemical compositions (hydroxyapatite (HA), beta-tricalcium phosphate (TCP) and a mixture of both with a HA/TCP ratio of 60/40 wt%). Using a subcutaneous implantation model in Wistar rats for up to 30 days, tissue reactions, including the induction of multinucleated giant cells and the extent of implantation bed vascularization, were assessed using histological and histomorphometrical analyses. The results showed that the chemical composition of the bone substitute material significantly influenced the cellular response. When compared to HA, TCP attracted significantly greater multinucleated giant cell formations within the implantation bed.Furthermore, the vascularization of the implantation bed of TCP was significantly higher than that of HA implantation beds. The biphasic bone substitute group combined the properties of both groups. Within the first 15 days, high giant cell formation and vascularization rates were observed, which were comparable to the TCP-group. However, after 15 days, the tissue reaction, i.e. the extent of multinucleated giant cell formation and vascularization, was comparable to the HA-group. In conclusion, the combination of both compounds HA and TCP may be a useful combination for generating a scaffold for rapid vascularization and integration during the early time points after implantation and for setting up a relatively slow degradation. Both of these factors are necessary for successful bone tissue regeneration.