OsSPL13 controls grain size in cultivated rice
OsSPL13 controls grain size in cultivated rice
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OsSPL13 控制栽培稻的颗粒大小
DOI：
10.1038/ng.3518
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发表时间：
2016-04-01
期刊：
影响因子：
30.8
通讯作者：
Han, Bin
中科院分区：
文献类型：
--
作者：
Si, Lizhen;Chen, Jiaying;Han, Bin
虽然遗传多样性在驯化中起着重要作用，但在水稻基因组中引起不同品种之间农艺学上重要差异的丰富的天然等位基因变异尚未得到充分探索。在这里，我们实现了一种方法，整合全基因组关联测试与功能分析的粒度在不同的水稻群体。我们报告了一个主要的数量性状位点，GLW7，编码植物特异性转录因子OsSPL13，正调控细胞的大小在粮食船体，导致增加水稻籽粒长度和产量。我们确定OsSPL13的5' UTR中的串联重复序列通过影响转录和翻译来改变其表达，并且OsSPL13的高表达与热带粳稻中的大粒相关。进一步分析表明，热带粳稻GLW7的大粒等位基因是在人工选择下从籼稻品种中渗入的。我们的研究表明，新的基因可以有效地识别全基因组关联数据的基础上。
Although genetic diversity has a cardinal role in domestication, abundant natural allelic variations across the rice genome that cause agronomically important differences between diverse varieties have not been fully explored. Here we implement an approach integrating genome-wide association testing with functional analysis on grain size in a diverse rice population. We report that a major quantitative trait locus, GLW7, encoding the plant-specific transcription factor OsSPL13, positively regulates cell size in the grain hull, resulting in enhanced rice grain length and yield. We determine that a tandem-repeat sequence in the 5' UTR of OsSPL13 alters its expression by affecting transcription and translation and that high expression of OsSPL13 is associated with large grains in tropical japonica rice. Further analysis indicates that the large-grain allele of GLW7 in tropical japonica rice was introgressed from indica varieties under artificial selection. Our study demonstrates that new genes can be effectively identified on the basis of genome-wide association data.