Biogenic and Synthetic Peptides with Oppositely Charged Amino Acids as Binding Sites for Mineralization.
Biogenic and Synthetic Peptides with Oppositely Charged Amino Acids as Binding Sites for Mineralization.
复制标题
DOI：
10.3390/ma10020119
复制
发表时间：
2017-01-28
期刊：
影响因子：
--
通讯作者：
Rothenstein D
中科院分区：
文献类型：
--
作者：
Lemloh ML;Altintoprak K;Wege C;Weiss IM;Rothenstein D
蛋白质通过与核酸、蛋白质和无机分子的特定相互作用来调节各种生物过程。无机杂化材料的产生，如软体动物的贝壳形成，是一个由蛋白质控制的矿化过程。此外，无机结合肽在非天然无机功能材料的生物矿化技术应用中具有很大的吸引力。然而，从生物系统和技术系统中识别矿物质结合多肽基序仍然是具有挑战性的。在这里，结合三种互补的方法来分析由交替带正负电荷的氨基酸组成的蛋白质基序：(I)天然生物矿化蛋白的筛选；(Ii)噬菌体展示衍生的无机结合多肽的选择；以及(Iii)基于烟草花叶病毒(TMV)的模板的矿化。TMV表面显示的不同的多肽基序对SiO_2矿化有重要影响。此外，在氧化锌和氧化锌结合肽中也发现了类似的基序，表明了一般的结合特征。这里提出的比较分析提出了新的问题，即是否存在基于酸性和碱性氨基酸的多肽与矿物相互作用的共同设计原则。
Proteins regulate diverse biological processes by the specific interaction with, e.g., nucleic acids, proteins and inorganic molecules. The generation of inorganic hybrid materials, such as shell formation in mollusks, is a protein-controlled mineralization process. Moreover, inorganic-binding peptides are attractive for the bioinspired mineralization of non-natural inorganic functional materials for technical applications. However, it is still challenging to identify mineral-binding peptide motifs from biological systems as well as for technical systems. Here, three complementary approaches were combined to analyze protein motifs consisting of alternating positively and negatively charged amino acids: (i) the screening of natural biomineralization proteins; (ii) the selection of inorganic-binding peptides derived from phage display; and (iii) the mineralization of tobacco mosaic virus (TMV)-based templates. A respective peptide motif displayed on the TMV surface had a major impact on the SiO2 mineralization. In addition, similar motifs were found in zinc oxide- and zirconia-binding peptides indicating a general binding feature. The comparative analysis presented here raises new questions regarding whether or not there is a common design principle based on acidic and basic amino acids for peptides interacting with minerals.