Integrated microfluidic UV absorbance detector with attomol-level sensitivity for BSA.
Integrated microfluidic UV absorbance detector with attomol-level sensitivity for BSA.
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DOI：
10.1039/b511766f
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发表时间：
2006-12
期刊：
影响因子：
6.1
通讯作者：
Likun Zhu;Cheng S. Lee;D. DeVoe
中科院分区：
文献类型：
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作者：
Likun Zhu;Cheng S. Lee;D. DeVoe
一个集成的紫外吸收检测系统采用了一种新的硅塑料技术无缝集成裸紫外光电二极管芯片到聚合物微流控系统已经开发出来。使用这种方法制造的检测平台表现出非常低的浓度和质量检测限分别低至15 nM和9.8阿莫尔，牛血清白蛋白（BSA）作为模型蛋白。除了提供高灵敏度外，还通过使用直接光电二极管集成实现了亚纳升检测体积。详细的制造方法，和系统的性能指标，包括检测限，检测体积，动态范围和线性度的报告。
An integrated UV absorbance detection system employing a novel silicon-in-plastic technology to seamlessly integrate bare UV photodiode chips into polymer microfluidic systems has been developed. Detection platforms fabricated using this approach exhibit exceptionally low concentration and mass detection limits down to 15 nM and 9.8 amol, respectively, for bovine serum albumin (BSA) as a model protein. In addition to providing high sensitivity, sub-nanoliter detection volumes are enabled by the use of direct photodiode integration. The fabrication methodology is detailed, and system performance metrics including detection limits, detection volume, dynamic range, and linearity are reported.