Technique for real-time tissue characterization based on scanning multispectral fluorescence lifetime spectroscopy (ms-TRFS)
Technique for real-time tissue characterization based on scanning multispectral fluorescence lifetime spectroscopy (ms-TRFS)
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DOI：
10.1364/boe.6.000987
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发表时间：
2015-01-01
影响因子：
3.4
通讯作者：
Marcu, Laura
中科院分区：
文献类型：
--
作者：
Ma, Dinglong;Bec, Julien;Marcu, Laura
我们报告了一种新的技术，用于连续采集，处理和显示荧光寿命，使实时组织诊断，通过一个单一的手持或活检光纤探头。建立了一种光子探测范围可调的扫描多光谱时间分辨荧光光谱仪（ms-TRFS），用于测量临床应用中常见的荧光强度动态变化。在ms-TRFS软件平台中实现了快速算法，提供高达15 Hz的荧光寿命值的连续显示。该技术的潜在应用，包括活检引导和手术切缘描绘在概念验证实验中得到证实。目前的结果显示了荧光寿命值的准确显示和实时区分不同的荧光标记物和组织类型（每个数据点< 100 ms）。(C)2015美国光学学会
We report a novel technique for continuous acquisition, processing and display of fluorescence lifetimes enabling real-time tissue diagnosis through a single hand held or biopsy fiber-optic probe. A scanning multispectral time-resolved fluorescence spectroscopy (ms-TRFS) with self-adjustable photon detection range was developed to account for the dynamic changes of fluorescence intensity typically encountered in clinical application. A fast algorithm was implemented in the ms-TRFS software platform, providing up to 15 Hz continuous display of fluorescence lifetime values. Potential applications of this technique, including biopsy guidance, and surgical margins delineation were demonstrated in proof-of-concept experiments. Current results showed accurate display of fluorescence lifetimes values and discrimination of distinct fluorescence markers and tissue types in real-time (< 100 ms per data point). (C) 2015 Optical Society of America