Single-unit recordings of arterial chemoreceptors from mouse petrosal ganglia in vitro
Single-unit recordings of arterial chemoreceptors from mouse petrosal ganglia in vitro
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DOI：
10.1152/jappl.2000.88.4.1489
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发表时间：
2000-04-01
影响因子：
3.3
通讯作者：
Rigual, R
中科院分区：
文献类型：
--
作者：
Donnelly, DF;Rigual, R
开发了一种制剂，可以记录小鼠颈动脉体的单单位化学受体活性。将麻醉小鼠斩首，取颈动脉体及窦神经、舌咽神经、岩神经节。暴露于胶原酶/胰蛋白酶后，清洗后的复合物被转移到记录室，在那里与含氧盐水混合。用玻璃吸电极搜索神经节诱发或自发的单位活动。单单位动作电位比记录噪声高57 +/- 10 (SE) （n = 16）个标准差，随机产生自发尖峰。将过饱和PO2降低到20 Torr附近，导致峰值活动从1.3 +/- 0.4 Hz增加到14.1 +/- 1.9 Hz （n = 16）。使用小鼠进行化学受体研究可能是有利的，因为靶向基因缺失在小鼠模型中得到了很好的发展，并且可能有助于解决有关化学转导机制的未解决问题。
A preparation was developed that allows for the recording of single-unit chemoreceptor activity from mouse carotid body in vitro. An anesthetized mouse was decapitated, and each carotid body was harvested, along with the sinus nerve, glossopharyngeal nerve, and petrosal ganglia. After exposure to collagenase/trypsin, the cleaned complex was transferred to a recording chamber where it was superfused with oxygenated saline. The ganglia was searched for evoked or spontaneous unit activity by using a glass suction electrode. Single-unit action potentials were 57 +/- 10 (SE) (n = 16) standard deviations above the recording noise, and spontaneous spikes were generated as a random process. Decreasing superfusate PO2 to near 20 Torr caused an increase in spiking activity from 1.3 +/- 0.4 to 14.1 +/- 1.9 Hz (n = 16). The use of mice for chemoreceptor studies may be advantageous because targeted gene deletions are well developed in the mouse model and may be useful in addressing unresolved questions regarding the mechanism of chemotransduction.