Use of comparative proteomics to identify the effects of creatine pyruvate on lipid and protein metabolism in broiler chickens
Use of comparative proteomics to identify the effects of creatine pyruvate on lipid and protein metabolism in broiler chickens
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DOI：
10.1016/j.tvjl.2012.01.034
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发表时间：
2012-08-01
影响因子：
2.2
通讯作者：
Zou, Sixiang
中科院分区：
文献类型：
--
作者：
Chen, Juan;Huang, Jianzhen;Zou, Sixiang
选取400只雄性鸡，研究了丙酮酸（Pyr）、肌酸丙酮酸（CrPyr）和肌酸（Cr）对肝脏线粒体及与脂质和蛋白质代谢相关的细胞质蛋白表达的影响。使用两步差速离心和密度梯度法完成线粒体纯化，并测定细胞器特异性标记酶的活性以评估线粒体的纯度。蛋白质通过双向电泳提取和分离，差异蛋白斑点通过基质辅助激光解吸/电离飞行时间质谱法进行鉴定。CrPyr通过下调脂肪分化相关蛋白，抑制ATP合酶的表达，降低胆固醇酯转移蛋白（CETP）的表达，从而降低高密度脂蛋白和甘油三酯（TG）水平（从而降低脂肪和胆固醇沉积），从而减少脂肪酸积累。CrPyr增加真核翻译起始因子（eIF） 2B、钙网蛋白（CRT）和eIF3a的表达，从而促进蛋白质合成。CrPyr上调脂肪酸结合蛋白的表达。细胞质提取物中的CETP和载脂蛋白A-IV，这些蛋白质加速脂肪酸和TG的分解，从而减少脂肪沉积。综上所述，CrPyr在脂肪分解和蛋白质合成中起着重要的作用，并且这种作用比Pyr和Cr的作用更明显。(C) 2012 Elsevier Ltd.。版权所有。
Four hundred male chickens were selected to study the effects of pyruvate (Pyr), creatine pyruvate (CrPyr) and creatine (Cr) on the expression of hepatic mitochondrial and cytoplasm proteins associated with lipid and protein metabolism. Mitochondrial purification was accomplished using the two-step differential centrifugation and density gradient method, and the activities of organelle-specific marker enzymes were determined to assess the purity of the mitochondria. Proteins were extracted and fractionated by two-dimensional electrophoresis and the differential protein spots were assessed by matrix-assisted laser desorption/ionization-time of flight mass spectrometry.CrPyr reduced fatty acid accumulation by down-regulating adipose differentiation-related protein, inhibited ATP synthase expression, and reduced cholesteryl ester transfer protein (CETP) expression, thus reducing the levels of high density lipoprotein and triglycerol (TG) levels (thereby lowering fat and cholesterol deposition). CrPyr increased the expression of eukaryotic translation initiation factor (eIF) 2B, calreticulin (CRT) and eIF3a, thus promoting protein synthesis. CrPyr up-regulated the expression of fatty acid-binding proteins. CETP and apolipoprotein A-IV in cytoplasmic extracts, and these proteins accelerated the decomposition of fatty acids and TG, thus reducing fat deposition. In conclusion, CrPyr plays an important role in lipolysis and protein synthesis, and this effect was more pronounced than was the effect of Pyr and Cr. (C) 2012 Elsevier Ltd. All rights reserved.