Functions of kinesin-1 motor protein in the telotrophic meroistic ovary of red flour beetle, <i>Tribolium castaneum</i>
Functions of kinesin-1 motor protein in the telotrophic meroistic ovary of red flour beetle, <i>Tribolium castaneum</i>
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赤拟谷盗端营养分期卵巢中驱动蛋白 1 的功能
DOI：
10.11416/jibs.89.1_017
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发表时间：
2020
影响因子：
--
通讯作者：
Keiko Takaki
中科院分区：
文献类型：
--
作者：
Taiki Sunada;Eiji Kotani;Yu Kaneko;Keiko Takaki
微管和马达蛋白被认为是维持卵母细胞细胞间极性的重要因素。利用RNA干扰技术研究了赤拟谷盗（Tribolium castaneum）运动蛋白基因驱动蛋白1（kinesin-1 motor protein）在卵巢中的功能。敲除该基因可诱导卵室期体细胞的排列紊乱。此外，击倒还导致卵室在几个阶段出现异常形状。RNAi不影响卵泡细胞的细胞分裂和调节卵泡细胞分裂和分化成柄细胞的Cut同源结构域蛋白的表达。我们的研究结果表明，驱动蛋白-1是必要的，在一个不同的方式，从在多营养卵巢的端营养卵巢的适当发展。
Microtubules and motor proteins are known as important factors in maintaining the intercellular polarity of oocytes in the polytrophic ovary. We examined the function of the kinesin-1 motor protein gene in the telotrophic ovary of Tribolium castaneum using RNA interference (RNAi). Knock down of the gene induced disarrangement of somatic follicle cells at the egg chamber stage in a specific manner. Furthermore, the knock down induced an abnormal shape of the egg chamber at several stages. The RNAi did not affect the cell division of follicle cells and expression of the Cut homeodomain protein that regulates follicle cell division and differentiation into stalk cells. Our results suggest that kinesin-1 is necessary in a different way for the proper development of the telotrophic ovary from that in the polytrophic ovary.