Retention, Molecular Evolution, and Expression Divergence of the Auxin/Indole Acetic Acid and Auxin Response Factor Gene Families in Brassica Rapa Shed Light on Their Evolution Patterns in Plants.
Retention, Molecular Evolution, and Expression Divergence of the Auxin/Indole Acetic Acid and Auxin Response Factor Gene Families in Brassica Rapa Shed Light on Their Evolution Patterns in Plants.
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DOI：
10.1093/gbe/evv259
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发表时间：
2015-12-31
影响因子：
3.3
通讯作者：
Hou X
中科院分区：
文献类型：
--
作者：
Huang Z;Duan W;Song X;Tang J;Wu P;Zhang B;Hou X
生长素/吲哚乙酸（Aux/IAAs）和生长素反应因子（ARF）是Aux信号网络的重要组成部分，参与植物的许多发育过程。研究这些基因的进化将为了解这些基因的分子进化与植物形态类型的增加之间的关系提供新的视角。我们构建的保留，结构，扩展，并在芜菁Aux/IAAs和ARF的表达模式及其在其他八种植物物种，包括藻类，苔藓植物，石松，被子植物的进化进行了比较分析。在B区共检测到33例ARF，包括1例ARF样蛋白（AL）和53例Aux/IAAs。芜菁基因组这些基因主要在13 Ma左右发生了分化。分裂后，没有Aux/IAA完全丢失，它们比ARF更优先保留。在陆生植物中，Aux/IAAs的扩增速度比ARF快，选择压力也相对较轻，ARF的稳定性提高。此外，BraIAAs比BraARF以更组织特异性的方式表达，并且在不同处理下的基因复制过程中表现出功能多样性，这增强了同源物之间的合作相互作用，以帮助植物适应复杂的环境。此外，ALs广泛存在，与ARF的关系更为密切，提示ALs可能是ARF的初始结构。我们的研究结果表明，Aux/IAAs的快速扩展和优先保留可能是由越来越复杂的形态类型在植物，甚至在陆地植物。同时，数据支持的假设，PB 1结构域在Aux/IAAs和ARF的起源中起着关键作用。
Auxin/indole acetic acids (Aux/IAAs) and auxin response factors (ARFs), major components of the Aux signaling network, are involved in many developmental processes in plants. Investigating their evolution will provide new sight on the relationship between the molecular evolution of these genes and the increasing morphotypes of plants. We constructed comparative analyses of the retention, structure, expansion, and expression patterns of Aux/IAAs and ARFs in Brassica rapa and their evolution in eight other plant species, including algae, bryophytes, lycophytes, and angiosperms. All 33 of the ARFs, including 1 ARF-like (AL) (a type of ARF-like protein) and 53 Aux/IAAs, were identified in the B. rapa genome. The genes mainly diverged approximately 13 Ma. After the split, no Aux/IAA was completely lost, and they were more preferentially retained than ARFs. In land plants, compared with ARFs, which increased in stability, Aux/IAAs expanded more rapidly and were under more relaxed selective pressure. Moreover, BraIAAs were expressed in a more tissue-specific fashion than BraARFs and demonstrated functional diversification during gene duplication under different treatments, which enhanced the cooperative interaction of homologs to help plants adapt to complex environments. In addition, ALs existed widely and had a closer relationship with ARFs, suggesting that ALs might be the initial structure of ARFs. Our results suggest that the rapid expansion and preferential retention of Aux/IAAs are likely paralleled by the increasingly complex morphotypes in Brassicas and even in land plants. Meanwhile, the data support the hypothesis that the PB1 domain plays a key role in the origin of both Aux/IAAs and ARFs.