Separation of mouse testis cells on a Celsep (TM) apparatus and their usefulness as a source of high molecular weight DNA or RNA.
Separation of mouse testis cells on a Celsep (TM) apparatus and their usefulness as a source of high molecular weight DNA or RNA.
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在 Celsep (TM) 装置上分离小鼠睾丸细胞及其作为高分子量 DNA 或 RNA 来源的用途。
DOI：
10.1002/mrd.1120120102
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Ponzetto,C
中科院分区：
文献类型：
--
作者：
Wolgemuth,DJ;Gizang-Ginsberg,E;Engelmyer,E;Gavin,BJ;Ponzetto,C
描述了一种自给式单位重力细胞分离器用于分离小鼠睾丸细胞群体。该设备为CELLEP™，使发生沉淀的单位面积最大化，减少了所使用的分离介质的量，并且具有很好的重复性。这样分离的细胞一直是分离DNA，特别是高分子量RNA的良好来源。
The use of a self‐contained unit‐gravity cell separation apparatus for separation of populations of mouse testicular cells is described. The apparatus, a Celsep™, maximizes the unit area over which sedimentation occurs, reduces the amount of separation medium employed, and is quite reproducible. Cells thus isolated have been good sources for isolation of DNA, and notably, high molecular weight RNA.