viking: identification and characterization of a second type IV collagen in Drosophila.
viking: identification and characterization of a second type IV collagen in Drosophila.
复制标题
viking：果蝇中第二种 IV 型胶原蛋白的鉴定和表征。
DOI：
10.1016/s0378-1119(97)00274-6
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发表时间：
1997
期刊：
影响因子：
3.5
通讯作者：
Dearolf,CR
中科院分区：
文献类型：
--
作者：
Yasothornsrikul,S;Davis,WJ;Cramer,G;Kimbrell,DA;Dearolf,CR
我们已经采取了增强子陷阱的方法来确定基因表达的造血细胞和组织的果蝇。我们进行了分子分析的两个P-元件插入菌株，报告基因在胚胎血细胞中的表达，菌株197和VikingICO。该分析已确定维京海盗编码IV型胶原蛋白基因α2（IV）。维京海盗基因座位于先前描述的编码胶原蛋白α1（IV）的DCg 1附近，并且方向相反。α2（IV）和α1（IV）胶原蛋白在结构上彼此非常相似，并且与脊椎动物IV型胶原蛋白非常相似。在早期发育中，维京海盗和DCg 1以相同的组织特异性模式转录，主要在血细胞和脂肪体细胞中。我们的研究结果表明，α1和α2胶原IV链可能有助于在果蝇的基底膜。这项工作也提供了一个更完整的基因解剖的IV型胶原蛋白分子及其在果蝇的发育功能的基础。
We have taken an enhancer trap approach to identify genes that are expressed in hematopoietic cells and tissues of Drosophila. We conducted a molecular analysis of two P-element insertion strains that have reporter gene expression in embryonic hemocytes, strain 197 and vikingICO. This analysis has determined that viking encodes a collagen type IV gene, α2(IV). The viking locus is located adjacent to the previously described DCg1, which encodes collagen α1(IV), and in the opposite orientation. The α2(IV) and α1(IV) collagens are structurally very similar to one another, and to vertebrate type IV collagens. In early development, viking and DCg1 are transcribed in the same tissue-specific pattern, primarily in the hemocytes and fat body cells. Our results suggest that both the α1 and α2 collagen IV chains may contribute to basement membranes in Drosophila. This work also provides the foundation for a more complete genetic dissection of collagen type IV molecules and their developmental function in Drosophila.