Essential developmental biology: a practical approach.
Essential developmental biology: a practical approach.
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基本发育生物学：实用方法。
DOI：
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发表时间：
1993
期刊：
影响因子：
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通讯作者：
P. Holland
中科院分区：
文献类型：
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作者：
C. Stern;P. Holland
第一部分基本技术--胚胎的获取和处理：果蝇胚胎，Zandy福布斯和Philip Ingham线虫胚胎，Lois G.埃德加和威廉B。木胚和幼虫的无脊椎动物后口类，尼古拉斯D。Holland和琳达Z.荷兰斑马鱼胚胎，Rachel M.作者：Ariel Ruis I. Altaba禽类胚胎，Claudio D.哺乳动物胚胎植入后，Gillian M。莫里斯-凯胚胎显微摄影的简单技巧。胸骨切开术组第2部分实验胚胎学：命运图和细胞谱系分析，Ariel Ruiz I。Altaba等的果蝇胚胎显微注射，Philip Ingham和Zandy的爪蟾福布斯细胞移植，Ariel Ruiz I. Altaba在禽类胚胎中的移植，Claudio D.斯特恩鸡肢芽，切里尔痒核移植，约翰B。Gordon laser ablation of cells，Judith Rumen et al. Part 3 Cellular techniques：studying cell movements in vivo by time-lapse video microscopy，Peter V. Thorogood and Dee Amanze induction assays in Xenopus，Ariel Ruiz I. Altaba培养的鸟类神经嵴细胞，Elisabeth Dupin和Nicole M.胚胎干细胞和基因靶向，Elizabeth J. Robertson和Gail R.马丁使用逆转录病毒载体引入基因，杰克普莱斯。第四部分分子技术：胚胎材料的免疫化学。Stern RNA酶保护测定，Ariel Ruiz I. Altaba cDNA文库的构建，Akira Kakizuka等逆转录聚合酶链反应分析基因表达，Peter Koopman用聚合酶链反应克隆基因，Peter W.H. Holland原位杂交，大卫G.威尔金森。附录：阶段表专业物品供应商。
Part 1 Basic techniques - obtaining and handling embryos: drosophila embryos, Zandy Forbes and Philip Ingham nematode embryos, Lois G. Edgar and William B. Wood embryos and larvae of invertebrate deuterostomes, Nicholas D. Holland and Linda Z. Holland zebrafish embryos, Rachel M. Warga Xenopus, Ariel Ruis I. Altaba avian embryos, Claudio D. Stern post-implantation of mammalian embryos, Gillian M. Morriss-Kay simple tips for photomicrography of embryos, Claudio D. Stern. Part 2 Experimental embryology: fate maps and cell lineage analysis, Ariel Ruiz I. Altaba et al micro-injection into drosophila embryos, Philip Ingham and Zandy Forbes cell transplantation in xenopus, Ariel Ruiz I. Altaba transplantation in avian embryos, Claudio D. Stern chick limb buds, Cheryll tickle nuclear transplantation, John B. Gordon laser ablation of cells, Judith Eisen et al. Part 3 Cellular techniques: studying cell movements in vivo by time-lapse video microscopy, Peter V. Thorogood and Dee Amanze induction assays in Xenopus, Ariel Ruiz I. Altaba culture of avian neural crest cells, Elisabeth Dupin and Nicole M. Le Douarin embryonic stem cells and gene targeting, Elizabeth J. Robertson and Gail R. Martin introduction of genes using retroviral vectors, Jack Price. Part 4 Molecular techniques: immonocytochemistry of embryonic material, Claudio D. Stern RNAase protection assays, Ariel Ruiz I. Altaba cDNA library construction, Akira Kakizuka et al analysis of gene expression by reverse transcription polymerase chain reaction, Peter Koopman cloning genes using the polymerase chain reaction, Peter W.H. Holland in situ hybridization, David G. Wilkinson. Appendices: stage tables suppliers of specialist items.