Vasopressin mRNA expression in individual magnocellular neuroendocrine cells of the supraoptic and paraventricular nucleus in response to water deprivation.
Vasopressin mRNA expression in individual magnocellular neuroendocrine cells of the supraoptic and paraventricular nucleus in response to water deprivation.
复制标题
视上核和室旁核个体大细胞神经内分泌细胞中加压素 mRNA 表达对缺水的反应。
DOI：
10.1159/000125881
复制
发表时间：
1991
影响因子：
4.1
通讯作者：
Hayward,JN
中科院分区：
文献类型：
--
作者：
Meeker,RB;Greenwood,RS;Hayward,JN
加压素神经内分泌功能涉及调节大细胞神经内分泌细胞的分泌和合成，但这两个过程的协调尚不清楚。为了探讨生理刺激与抗利尿激素mRNA水平之间的时间关系，我们测量了缺水过程中视上核和室旁核单个大细胞神经元内抗利尿激素mRNA的含量。通过原位杂交分析[125I] dctp标记的抗利尿激素mRNA特异性寡核苷酸的自显影银颗粒密度，揭示了大细胞神经内分泌细胞中静息抗利尿激素mRNA水平的多样性和对缺水的差异反应。在缺水期间，室旁核的抗利尿激素mRNA含量迅速增加，潜伏期短，增量反应大于视上核。结合原位杂交和免疫细胞化学的双标记实验鉴定了一群抗利尿激素免疫反应细胞，它们维持了非常低的抗利尿激素mRNA的基础水平。这些细胞的位置与缺水期间银颗粒密度增加的位置相关。抗利尿激素大细胞神经元的一个亚群没有显示出高水平的抗利尿激素mRNA，这表明所有细胞对缺水的反应并不相同。这些抗利尿激素mRNA的表达模式表明抗利尿激素神经内分泌细胞的功能亚群的存在，这可能反映了视上核和室旁核传入输入的刺激特异性模式。
Vasopressin neuroendocrine function involves the regulation of both secretion and synthesis from magnocellular neuroendocrine cells but the coordination of these two processes is poorly understood. To explore the temporal relationship between physiological stimulation and vasopressin mRNA levels we measured vasopressin mRNA content within individual magnocellular neurons of the supraoptic and paraventricular nucleus during the course of water deprivation. Analysis of autora-diographic silver grain densities from in situ hybridization of an [125I]dCTP-labeled oligonucleotide specific for vasopressin mRNA revealed a wide variety of resting vasopressin mRNA levels and differential responses to water deprivation in the magnocellular neuroendocrine cells. During water deprivation, the vasopressin mRNA content of the paraventricular nucleus increases rapidly and with shorter latency and greater incremental response than the supraoptic nucleus. Double-labeling experiments with combined in situ hybridization and immunocytochemistry identified a population of vasopressin immunoreactive cells which maintain very low basal levels of vasopressin mRNA. The location of these cells correlates with the location of increased silver grain densities during water deprivation. One subset of vasopressin magnocellular neurons failed to show high levels of vasopressin mRNA, indicating that all cells are not equally responsive to water deprivation. These patterns of vasopressin mRNA expression suggest the presence of functional subpopulations of vasopressin neuroendocrine cells which may reflect stimulus-specific patterns of afferent input to the supraoptic and paraventricular nucleus.