Vesicle endocytosis requires dynamin-dependent GTP hydrolysis at a fast CNS synapse
Vesicle endocytosis requires dynamin-dependent GTP hydrolysis at a fast CNS synapse
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DOI：
10.1126/science.1103631
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发表时间：
2005-01-07
期刊：
影响因子：
56.9
通讯作者：
Takahashi, T
中科院分区：
文献类型：
--
作者：
Yamashita, T;Hige, T;Takahashi, T
在脑干切片上，用电容测量的方法研究了囊泡内吞作用的分子依赖性。肉毒杆菌毒素E的端内负荷显示，“接吻即跑”的快速电容瞬变与递质释放无关。与释放相关的电容。内吞时间常数为10到25秒，这取决于胞吐的大小。突触前负荷的非水解性鸟苷，5‘-三磷酸(GTP)类似物GTP-Gamma或Dynamin-1 Pro富含结构域的多肽可取消内吞作用。这些化合物对胞吐作用没有立竿见影的效果，但会导致依赖于使用的胞吐作用减弱。因此，鸟苷三磷酸酶Dynamin-1对于这个快速中枢神经系统(CNS)突触的囊泡内吞是必不可少的。
Molecular dependence of vesicular endocytosis was investigated with capacitance measurements at the calyx of Held terminal in brainstem slices. Intraterminal loading of botulinum toxin E revealed that the rapid capacitance transient implicated as "kiss-and-run" was unrelated to transmitter release. The release-related capacitance. change decayed with an endocytotic time constant of 10 to 25 seconds, depending on the magnitude of exocytosis. Presynaptic loading of the nonhydrolyzable guanosine, 5'-triphosphate (GTP) analog GTPgammaS or dynamin-1 proline-rich domain peptide abolished endocytosis. These compounds had no immediate effect on exocytosis, but caused a use-dependent rundown of exocytosis. Thus, the guanosine triphosphatase dynamin-1 is indispensable for vesicle endocytosis at this fast central nervous system (CNS) synapse.