Responses of odontoblasts to cavity preparation in rat molars as demonstrated by immunocytochemistry for heat shock protein (Hsp) 25.
Responses of odontoblasts to cavity preparation in rat molars as demonstrated by immunocytochemistry for heat shock protein (Hsp) 25.
复制标题
热休克蛋白 (Hsp) 25 的免疫细胞化学证实了成牙本质细胞对大鼠磨牙空洞制备的反应。
DOI：
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发表时间：
2001
影响因子：
--
通讯作者：
Takeyasu Maeda
中科院分区：
文献类型：
--
作者：
Hayato Ohshima;K. Nakakura‐Ohshima;Hitoshi Yamamoto;Takeyasu Maeda
采用热休克蛋白（Hsp） 25免疫细胞化学方法研究成牙细胞对大鼠磨牙造腔的反应。在未治疗的对照牙中，在成牙细胞的细胞体及其牙本质内的过程中发现了强烈的热休克蛋白25免疫反应性。hsp25免疫染色和罗丹明标记切片的共聚焦显微镜显示，免疫反应性成牙细胞在其细胞质周围和整个过程中被强烈标记为phalloidin，但对phalloidin的反应仅限于牙本质的内半部分。空腔制备引起受伤的成牙本质和预备牙本质之间的水肿反应，以及珠状肿胀和成牙本质突的连续破坏。在准备好腔体后，水肿病变下的成牙本质细胞立即表现出对热休克蛋白25的免疫反应，随后在手术后12小时从牙髓-牙本质边界完全消失。然而，没有明显细胞质过程的圆形细胞继续具有免疫反应性，表明部分成牙髓细胞在制备刺激下存活。沿着牙髓-牙本质边界出现了大量的phalloidin反应性但hsp25免疫阴性的细胞，并将其过程深入到暴露的牙本质小管，可能属于免疫能力细胞谱系。术后72 h，新分化的具有热休克蛋白25免疫反应性的成牙本质细胞排列在牙髓-牙本质边界。这些结果表明，热休克蛋白25免疫反应性表达的时间变化过程反映了成牙细胞的再生过程，表明该蛋白是分化成牙细胞的有用标记物质。
Responses of odontoblasts to cavity preparation in rat molars were investigated by immunocytochemistry for heat shock protein (Hsp) 25. In untreated control teeth, intense Hsp 25-immunoreactivity was found in the cell bodies of odontoblasts and their processes within the predentin. Confocal microscopy of Hsp 25-immunostained and rhodamine-labeled sections revealed that the immunoreactive odontoblasts were intensely labeled for phalloidin at the periphery of their cytoplasm and throughout their processes, but the reaction for phalloidin was limited within the inner half of the dentin. Cavity preparation caused an edematous reaction between the injured odontoblasts and predentin as well as a beaded swelling and successive destruction of the odontoblast processes. Immediately after cavity preparation, the odontoblasts beneath the edematous lesion showed an immunoreactivity for Hsp 25, which subsequently disappeared completely from the pulp-dentin border by 12 h after the operation. However, round cells without apparent cytoplasmic processes continued to be immunoreactive, suggesting the survival of a part of the odontoblasts against preparation stimuli. Numerous phalloidin-reactive but Hsp 25-immunonegative cells appeared along the pulp-dentin border and extended their processes deep into the exposed dentinal tubules, probably categorized in a lineage of immunocompetent cells. By postoperative 72 h, newly differentiated odontoblasts with Hsp 25-immunoreactivity were arranged at the pulp-dentin border. These findings indicate that the time course of changes in the expression of Hsp 25-immunoreactivity reflects the regeneration process of odontoblasts, and suggest that this protein is a useful marker substance for differentiated odontoblasts.
大鼠磨牙空腔准备和伤口愈合后原代成牙本质细胞和替代成牙本质细胞的超微结构和放射自显影分析。
DOI：
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发表时间：
1992
期刊：
Proceedings of the Finnish Dental Society. Suomen Hammaslaakariseuran toimituksia
影响因子：
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作者：
ChiegoJr,DJ
通讯作者：
ChiegoJr,DJ
DOI：
10.1016/s0021-9258(20)80512-2
发表时间：
1993-11
期刊：
The Journal of biological chemistry
影响因子：
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作者：
J. Lavoie;E. Hickey;L. Weber;J. Landry
通讯作者：
J. Lavoie;E. Hickey;L. Weber;J. Landry