Elevated expression level of long noncoding RNA MALAT-1 facilitates cell growth, migration and invasion in pancreatic cancer
Elevated expression level of long noncoding RNA MALAT-1 facilitates cell growth, migration and invasion in pancreatic cancer
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长链非编码 RNA MALAT-1 表达水平升高促进胰腺癌细胞生长、迁移和侵袭。
DOI：
10.3892/or.2014.3518
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发表时间：
2014-12-01
期刊：
影响因子：
4.2
通讯作者：
Wang, Liwei
中科院分区：
文献类型：
--
作者：
Jiao, Feng;Hu, Hai;Wang, Liwei
胰腺癌是最具侵袭性的实体恶性肿瘤之一，生存率低。最近的研究表明，长链非编码RNA（lncRNA）转移相关肺腺癌转录本-1（MALAT-1）的高表达水平与几种实体瘤相关。然而，其在胰腺癌中的潜在分子机制及其临床意义仍有待阐明。在本研究中，我们的研究结果表明，MALAT-1表达水平上调胰腺癌组织与相邻的非癌对照。MALAT-1在胰腺癌细胞系中的表达水平均高于胰腺导管上皮细胞。进一步的功能分析显示MALAT-1的下调可以抑制肿瘤细胞的增殖，降低细胞的迁移和侵袭能力。其作用机制可能与诱导G2/M期细胞阻滞、促进细胞凋亡、抑制上皮-间质转化和降低肿瘤干细胞样特性有关。总之，这项研究表明，MALAT-1可能作为一个致癌的lncRNA参与胰腺癌的恶性表型。因此，它可能被用作潜在的治疗靶点。
Pancreatic cancer is one of the most aggressive solid malignancies with a dismal survival rate. Recent studies have shown that high expression levels of long noncoding RNA (lncRNA) metastasis-associated lung adenocarcinoma transcript-1 (MALAT-1) correlate with several solid tumors. However, the underlying molecular mechanisms and its clinical significance in pancreatic cancer remain to be elucidated. In the present study, our results showed that MALAT-1 expression levels were upregulated in pancreatic cancer tissues compared with adjacent noncancerous controls. Consistently, higher expression level of MALAT-1 was found in all seven pancreatic cancer cell lines relative to the human pancreatic ductal epithelial cell. Further function analysis revealed that downregulation of MALAT-1 could inhibit tumor cell proliferation and decrease cell migration and invasion in vitro. The underlying mechanisms are possibly involved in inducing G2/M cell cycle arrest, promoting cell apoptosis, suppressing epithelial-mesenchymal transition and reducing cancer stem-like properties. In conclusion, this study indicated that MALAT-1 may serve as an oncogenic lncRNA that is involved in malignancy phenotypes of pancreatic cancer. Therefore, it may be used as a potential therapeutic target.