Rim101-upregulated Fets contribute to dark pigment formation in gray cells of Candida albicans
Rim101-upregulated Fets contribute to dark pigment formation in gray cells of Candida albicans
复制标题
DOI：
10.1093/abbs/gmab142
复制
发表时间：
2021-10-02
影响因子：
3.7
通讯作者：
Chen, Jiangye
中科院分区：
文献类型：
--
作者：
Dai, Baodi;Xu, Yinxing;Chen, Jiangye
人们早就知道白色念珠菌会在白色和不透明的阶段之间切换；然而，第三种细胞类型，被称为“灰色”表型，最近被表征。三种表型具有不同的群体形态，白细胞形成白色的群体，不透明和灰色的细胞形成深色的群体。我们之前的研究表明，wor1上调的铁氧化酶（Fets）作为色素多铜氧化酶，调节不透明细胞中深色色素黑色素的产生。在这项研究中，我们证明了Fets也有助于灰色菌落中深色色素的形成，但以wor1独立的方式。在一些富培养基中，WOR1和EFG1的缺失都锁定了灰色表型的细胞。然而，efgl/efgl wor1/wor1突变体可以在最小的培养基中根据环境pH值在白色和灰色之间切换。具体来说，突变体细胞在pH值为4.5时表现为白色表型，但在pH值为7.5时变为灰色表型。与表型转换一致，Fets的表达和黑色素的产生也受到环境pH的调节。突变体中Rim101-405等位基因的异位表达使pH限制被绕过，并促进酸性培养基中灰色细胞的形成。我们的数据表明，rim101上调的Fets有助于灰色细胞中深色色素的形成。
Candida albicans has long been known to switch between white and opaque phases; however, a third cell type, referred to as the 'gray' phenotype, was recently characterized. The three phenotypes have different colonial morphologies, with white cells forming white-colored colonies and opaque and gray cells forming dark-colored colonies. We previously showed that Wor1-upregulated ferroxidases (Fets) function as pigment multicopper oxidases that regulate the production of dark-pigmented melanin in opaque cells. In this study, we demonstrated that Fets also contributed to dark pigment formation in gray colonies but in a Wor1-independent manner. Deletion of both WOR1 and EFG1 locked cells in the gray phenotype in some rich media. However, the efgl/efgl wor1/wor1 mutant could switch between white and gray in minimal media depending on the ambient pH. Specifically, mutant cells exhibited the white phenotype at pH 4.5 but switched to gray at pH 7.5. Consistent with phenotype switching, Fets expressions and melanin production were also regulated by ambient pH. Ectopic expression of the Rim101-405 allele in the mutant enabled the pH restriction to be bypassed and promoted gray cell formation in acidic media. Our data suggest that Rim101-upregulated Fets contribute to dark pigment formation in the gray cells.