Multiplexed dendritic targeting of α calcium calmodulin-dependent protein kinase II, neurogranin, and activity-regulated cytoskeleton-associated protein RNAs by the A2 pathway
Multiplexed dendritic targeting of α calcium calmodulin-dependent protein kinase II, neurogranin, and activity-regulated cytoskeleton-associated protein RNAs by the A2 pathway
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DOI：
10.1091/mbc.e07-09-0914
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发表时间：
2008-05-01
影响因子：
3.3
通讯作者：
Carson, John H.
中科院分区：
文献类型：
--
作者：
Gao, Yuanzheng;Tatavarty, Vedakumar;Carson, John H.
在神经元中，许多不同的 RNA 靶向树突，其中编码蛋白的局部表达介导学习和记忆过程中的突触可塑性。目前尚不清楚是否每个 RNA 都遵循单独的运输途径，或者多个 RNA 是否通过相同的途径靶向树突。在这里，我们发现编码α钙钙调蛋白依赖性蛋白激酶II、神经粒蛋白和活性调节细胞骨架相关蛋白的RNA被共同组装成相同的RNA颗粒，并通过相同的顺式/反式决定簇（异质核核糖核蛋白[hnRNP] A2反应元件和hnRNP A2）靶向树突，这些决定簇通过少突胶质细胞中的A2途径介导髓鞘碱性蛋白RNA的树突靶向。通过同一途径针对不同RNA的多重树突代表了协调突触基因表达的新组织原则。
In neurons, many different RNAs are targeted to dendrites where local expression of the encoded proteins mediates synaptic plasticity during learning and memory. It is not known whether each RNA follows a separate trafficking pathway or whether multiple RNAs are targeted to dendrites by the same pathway. Here, we show that RNAs encoding alpha calcium calmodulin-dependent protein kinase II, neurogranin, and activity-regulated cytoskeleton-associated protein are coassembled into the same RNA granules and targeted to dendrites by the same cis/trans-determinants ( heterogeneous nuclear ribonucleoprotein [hnRNP] A2 response element and hnRNP A2) that mediate dendritic targeting of myelin basic protein RNA by the A2 pathway in oligodendrocytes. Multiplexed dendritic targeting of different RNAs by the same pathway represents a new organizing principle for coordinating gene expression at the synapse.