A tertiary interaction that links active-site domains to the 5′ splice site of a group II intron
A tertiary interaction that links active-site domains to the 5′ splice site of a group II intron
复制标题
将活性位点结构域连接到 II 组内含子的 5 剪接位点的三级相互作用
作者：
M. Boudvillain;A. D. Lencastre;A. Pyle
Group II introns are self-splicing RNAs that are commonly found in the genes of plants, fungi, yeast and bacteria. Little is known about the tertiary structure of group II introns, which are among the largest natural ribozymes. The most conserved region of the intron is domain 5 (D5), which, together with domain 1 (D1), is required for all reactions catalysed by the intron. Despite the importance of D5, its spatial relationship and tertiary contacts to other active-site constituents have remained obscure. Furthermore, D5 has never been placed directly at a site of catalysis by the intron. Here we show that a set of tertiary interactions (λ–λ′) links catalytically essential regions of D5 and D1, creating the framework for an active-site and anchoring it at the 5′ splice site. Highly conserved elements similar to components of the λ–λ′ interaction are found in the eukaryotic spliceosome.
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DOI：
10.1073/pnas.94.7.2903
发表时间：
1997
影响因子：
11.1
作者：
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通讯作者：
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影响因子：
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作者：
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DOI：
--
发表时间：
1995
期刊：
RNA (New York, N.Y.)
影响因子：
--
作者：
Yu,YT;Maroney,PA;Darzynkiwicz,E;Nilsen,TW
通讯作者：
Nilsen,TW
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DOI：
--
发表时间：
1995
期刊：
RNA (New York, N.Y.)
影响因子：
--
作者：
Chin,K;Pyle,AM
通讯作者：
Pyle,AM
影响因子：
6.8
作者：
Qin, PZ;Pyle, AM
通讯作者：
Pyle, AM